Guo Cun, Wang Qi, Li Zhiyuan, Sun Jinhao, Zhang Zenglin, Li Xiaoxu, Guo Yongfeng
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China.
Front Genet. 2021 Apr 27;12:670794. doi: 10.3389/fgene.2021.670794. eCollection 2021.
The inflorescence deficient in abscission-like () genes have been shown to play critical roles in floral organ abscission, lateral root formation and various stress responses in . The gene family has been characterized in a number of plant species, while limited information is available about genes of tobacco. In the current study, 15 NtIDL members were identified in the tobacco genome, and were classified into six groups together with IDL members from other species. Evolution analysis suggested that the NtIDL members form group VI might have originated from duplication events. Notably, NtIDL06 shared high similarities with AtIDA in the EPIP sequence, and its encoding gene was highly expressed in the abscission zone of flowers at late developmental stages, implying that NtIDL06 might regulate tobacco flower abscission. In addition, the results from -elements analysis of promoters and expression after stress treatments suggested that members might be involved in various stress responses of tobacco. The results from this study provide information for further functional analysis related to flower abscission and stress responses of genes.
缺乏类脱落（）基因的花序已被证明在花器官脱落、侧根形成以及植物的各种应激反应中起关键作用。该基因家族已在许多植物物种中得到表征，而关于烟草的基因信息有限。在本研究中，在烟草基因组中鉴定出15个NtIDL成员，并与来自其他物种的IDL成员一起分为六组。进化分析表明，形成第六组的NtIDL成员可能起源于复制事件。值得注意的是，NtIDL06在EPIP序列中与AtIDA具有高度相似性，其编码基因在发育后期的花脱落区高度表达，这意味着NtIDL06可能调控烟草花的脱落。此外，启动子的顺式作用元件分析结果和应激处理后的表达结果表明，成员可能参与烟草的各种应激反应。本研究结果为进一步对基因的花脱落和应激反应相关功能分析提供了信息。
