Faggioni A, Zompetta C, Grimaldi S, Barile G, Frati L, Lazdins J
Science. 1986 Jun 20;232(4757):1554-6. doi: 10.1126/science.3012779.
In many viral infections the host cell carries the viral genome without producing viral particles, a phenomenon known as viral latency. The cellular mechanisms by which viral latency is maintained or viral replication is induced are not known. The modulation of intracellular calcium concentrations by calcium ionophores induced Epstein-Barr viral antigens in lymphoblastoid cell lines that carry the virus. When calcium ionophores were used in conjunction with direct activators of protein kinase C (12-O-tetradecanoyl phorbol-13-acetate and a synthetic diacylglycerol), a greater induction of viral antigens was observed than with either agent alone. Activation of protein kinase C may be required for the expression of the viral genome.
在许多病毒感染中，宿主细胞携带病毒基因组却不产生病毒颗粒，这种现象被称为病毒潜伏。维持病毒潜伏或诱导病毒复制的细胞机制尚不清楚。钙离子载体对细胞内钙浓度的调节可在携带该病毒的淋巴母细胞系中诱导出爱泼斯坦-巴尔病毒抗原。当钙离子载体与蛋白激酶C的直接激活剂（12-O-十四烷酰佛波醇-13-乙酸酯和一种合成二酰基甘油）联合使用时，观察到病毒抗原的诱导作用比单独使用任何一种试剂时都更强。蛋白激酶C的激活可能是病毒基因组表达所必需的。
