Guo Lihua, Yin Min, Wang Yixuan
Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.
Oncol Lett. 2018 Sep;16(3):3842-3848. doi: 10.3892/ol.2018.9118. Epub 2018 Jul 10.
Bladder cancer (BC) is a prevalent cancer, which arises from the epithelial lining of the urinary bladder. CAMP-response element binding protein (CREB1) acts as a transcription factor, which regulates cell transcription through phosphorylation and dephosphorylation. The purpose of this study was to explore how miR-122 worked in BC on cell proliferation and invasion. RT-qPCR was applied to evaluate the mRNA levels of CREB1 and miR-122 in BC. CCK-8 and Transwell assays were employed to determine the migratory and invasive abilities. Dual luciferase reporter assay was applied to verify miR-122 targeting CREB1 in BC. CREB1 was upregulated in bladder tissues and T24, UM-UC-3 and J82 cells, while miR-122 upregulated and had negative correlation with CREB1. Moreover, knockdown of CREB1 inhibited cell proliferative and invasive capacities. In addition, CREB1 was directly targeted by miR-122 in BC and regulated its expression. We discovered that CREB1 could reverse partially the function of miR-122 on cell proliferation and invasion. CREB1 was mediated by miR-122, and regulated cell proliferation and invasiveness. The newly identified miR-122/CREB1 axis provides novel insight into the pathogenesis of BC.
膀胱癌（BC）是一种常见的癌症，起源于膀胱的上皮内层。环磷酸腺苷反应元件结合蛋白（CREB1）作为一种转录因子，通过磷酸化和去磷酸化调节细胞转录。本研究的目的是探讨miR-122在膀胱癌中对细胞增殖和侵袭的作用机制。采用逆转录-定量聚合酶链反应（RT-qPCR）评估膀胱癌中CREB1和miR-122的mRNA水平。采用细胞计数试剂盒-8（CCK-8）和Transwell实验检测细胞的迁移和侵袭能力。应用双荧光素酶报告基因实验验证miR-122在膀胱癌中靶向CREB1。CREB1在膀胱组织以及T24、UM-UC-3和J82细胞中表达上调，而miR-122表达上调且与CREB1呈负相关。此外，敲低CREB1可抑制细胞的增殖和侵袭能力。另外，在膀胱癌中miR-122直接靶向CREB1并调节其表达。我们发现CREB1可部分逆转miR-122对细胞增殖和侵袭的作用。CREB1受miR-122介导，调控细胞增殖和侵袭能力。新发现的miR-122/CREB1轴为膀胱癌的发病机制提供了新的见解。
