Georgiev Vasil N, Grafmüller Andrea, Bléger David, Hecht Stefan, Kunstmann Sonja, Barbirz Stefanie, Lipowsky Reinhard, Dimova Rumiana
Department of Theory and Bio-Systems Max Planck Institute of Colloids and Interfaces Science Park Golm 14424 Potsdam Germany.
Department of Chemistry & IRIS Adlershof Humboldt-Universität zu Berlin Brook-Taylor-Str. 2 12489 Berlin Germany.
Adv Sci (Weinh). 2018 Jun 5;5(8):1800432. doi: 10.1002/advs.201800432. eCollection 2018 Aug.
Biomembranes are constantly remodeled and in cells, these processes are controlled and modulated by an assortment of membrane proteins. Here, it is shown that such remodeling can also be induced by photoresponsive molecules. The morphological control of giant vesicles in the presence of a water-soluble -tetrafluoroazobenzene photoswitch (F-azo) is demonstrated and it is shown that the shape transformations are based on an increase in membrane area and generation of spontaneous curvature. The vesicles exhibit budding and the buds can be retracted by using light of a different wavelength. In the presence of F-azo, the membrane area can increase by more than 5% as assessed from vesicle electrodeformation. To elucidate the underlying molecular mechanism and the partitioning of F-azo in the membrane, molecular dynamics simulations are employed. Comparison with theoretically calculated shapes reveals that the budded shapes are governed by curvature elasticity, that the spontaneous curvature can be decomposed into a local and a nonlocal contribution, and that the local spontaneous curvature is about 1/(2.5 µm). The results show that exo- and endocytotic events can be controlled by light and that these photoinduced processes provide an attractive method to change membrane area and morphology.
生物膜不断地进行重塑，在细胞中，这些过程由多种膜蛋白控制和调节。在此，研究表明这种重塑也可由光响应分子诱导。证明了在水溶性 - 四氟偶氮苯光开关（F - 偶氮）存在下对巨型囊泡的形态控制，并且表明形状转变基于膜面积的增加和自发曲率的产生。囊泡呈现出出芽现象，并且可以通过使用不同波长的光使芽缩回。在F - 偶氮存在下，根据囊泡电变形评估，膜面积可增加超过5%。为了阐明潜在的分子机制以及F - 偶氮在膜中的分配情况，采用了分子动力学模拟。与理论计算形状的比较表明，出芽形状受曲率弹性支配，自发曲率可分解为局部和非局部贡献，并且局部自发曲率约为1/(2.5 µm)。结果表明，胞吐和胞吞事件可由光控制，并且这些光诱导过程为改变膜面积和形态提供了一种有吸引力的方法。
