Zhang Tingting, Zeng Xiangxing, Zeng Zhu, Hu Zuquan, Yang Feiyu
Engineering Research Center of Medical Biotechnology/Key Laboratory of Biology and Medical Engineering, Guizhou Medical University, Guiyang, 550000, Guizhou, People's Republic of China.
Immune Cells and Antibody Engineering Research Center of Guizhou Province/Cell and Gene Engineering Innovative Research Groups of Guizhou Province, Guizhou Medical University, Guiyang, 550000, Guizhou, People's Republic of China.
Arch Virol. 2018 Dec;163(12):3427-3431. doi: 10.1007/s00705-018-3976-6. Epub 2018 Aug 20.
In this study, we describe a novel mycovirus isolated from Ustilaginoidea virens strain GZ-2, which was designated "Ustilaginoidea virens nonsegmented virus 2" (UvNV-2). The genome of UvNV-2 contains two overlapping open reading frames (ORFs). ORF1 encodes an unknown protein, and ORF2 encodes a putative RNA-dependent RNA polymerase (RdRp), which is most closely related to that of Purpureocillium lilacinum nonsegmented virus 1 (PINV-1) and is likely to be expressed by a + 1 ribosomal frameshift within the sequence CCC_UUU_UAG. A phylogenetic analysis of the RdRp of UvNV-2 showed that UvNV-2 is an unclassified mycovirus.
在本研究中,我们描述了一种从稻曲病菌株GZ-2中分离出的新型真菌病毒,将其命名为“稻曲病无节段病毒2”(UvNV-2)。UvNV-2的基因组包含两个重叠的开放阅读框(ORF)。ORF1编码一种未知蛋白质,ORF2编码一种假定的RNA依赖RNA聚合酶(RdRp),该酶与淡紫拟青霉无节段病毒1(PINV-1)的RdRp关系最为密切,可能通过序列CCC_UUU_UAG内的+1核糖体移码进行表达。对UvNV-2的RdRp进行的系统发育分析表明,UvNV-2是一种未分类的真菌病毒。