Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Arch Virol. 2013 Nov;158(11):2415-9. doi: 10.1007/s00705-013-1742-3. Epub 2013 Jun 4.
From the plant pathogen Ustilaginoidea virens, four double-stranded RNA (dsRNA) segments designated Uv-dsRNA1, -2, -3, and -4 were isolated, cloned, and sequenced. Uv-dsRNA1 (1775 bp) and -2 (1588 bp) potentially encode an RNA-dependent RNA polymerase (RdRp) and a viral coat protein (CP), respectively. Since the RdRp and CP sequences encoded by Uv-dsRNA1 and -2, respectively, are most closely related to, but clearly distinct from, those of viruses of the genus Partitivirus, they appear to be the two genome segments of a new partitivirus, for which the name Ustilaginoidea virens partitivirus 1 is proposed. In contrast, Uv-dsRNA3 (1352 bp) did not share significant sequence similarity with GenBank sequences, and the ORF of Uv-dsRNA4 (1119 bp) was only 32 % identical to a functionally unknown protein (GaRVMS2s3gp1) encoded by Gremmeniella abietina RNA virus MS2.
从真菌病原菌 Ustilaginoidea virens 中分离、克隆并测序了四个双链 RNA (dsRNA) 片段，分别命名为 Uv-dsRNA1、-2、-3 和 -4。Uv-dsRNA1（1775bp）和 -2（1588bp）分别可能编码 RNA 依赖性 RNA 聚合酶（RdRp）和病毒外壳蛋白（CP）。由于 Uv-dsRNA1 和 -2 编码的 RdRp 和 CP 序列与节肢介体病毒属的病毒最为密切相关，但又明显不同，因此它们似乎是一种新的二分体病毒的两个基因组片段，建议将其命名为 Ustilaginoidea virens 二分体病毒 1。相比之下，Uv-dsRNA3（1352bp）与 GenBank 序列没有显著的序列相似性，而 Uv-dsRNA4（1119bp）的 ORF 与功能未知的蛋白（GaRVMS2s3gp1）只有 32%的同一性，该蛋白由 Gremmeniella abietina RNA 病毒 MS2 编码。
