Keller P M, Davison A J, Lowe R S, Bennett C D, Ellis R W
Virology. 1986 Jul 15;152(1):181-91. doi: 10.1016/0042-6822(86)90383-1.
The genome of varicella-zoster virus (VZV) encodes three major families of glycoproteins (gpI, gpII, and gpIII). mRNA from VZV-infected cells was hybrid selected using a library of VZV recombinant plasmids and translated in vitro; polypeptide products were immunoprecipitated by polyclonal monospecific guinea pig antibodies to gpII. The mRNA encoding a 100-kD polypeptide precipitable by anti-gpII antibodies mapped to the HindIII D fragment near the center of the UL region. DNA sequence analysis of this region of the VZV genome revealed a 2.6-kbp open reading frame (ORF) potentially encoding a 98-kDa polypeptide possessing the characteristics of a glycoprotein. The 100-kDa polypeptide was specified by mRNA isolated by hybrid selection using a plasmid containing part of the 2.6-kbp ORF, and immunoprecipitation of this protein by anti-gpII antibodies and by convalescent zoster serum was blocked specifically by purified gpII. We conclude that the 2.6-kbp ORF encodes gpII. The imputed primary amino acid sequence of gpII shows a high degree of homology to that of herpes simplex virus type 1 (HSV-1) gB, a result consistent with the equivalent map locations of the respective genes in the HSV and VZV genomes and with the recently reported serological cross-reactivity of HSV-1 gB and VZV gpII. Unlike the mature gene products of gB, those of gpII have been described as a pair of glycoproteins with approximate molecular weights of 60 kDa in reducing gels, products of a single glycoprotein species with approximate mol mass of 125-140 kDa in nonreducing gels. Amino-terminal sequences of purified gpII were determined and compared to the imputed amino acid sequence. This comparison implies that the primary translational product is cleaved approximately into halves in vivo and suggests that mature gpII is a disulfide-linked heterodimer.
水痘带状疱疹病毒（VZV）的基因组编码三类主要的糖蛋白家族（糖蛋白I、糖蛋白II和糖蛋白III）。使用VZV重组质粒文库对VZV感染细胞的mRNA进行杂交筛选并在体外进行翻译；多肽产物用针对糖蛋白II的多克隆单特异性豚鼠抗体进行免疫沉淀。编码可被抗糖蛋白II抗体沉淀的100-kD多肽的mRNA定位于UL区域中心附近的HindIII D片段。对VZV基因组该区域的DNA序列分析揭示了一个2.6-kbp的开放阅读框（ORF），其可能编码一种具有糖蛋白特征的98-kDa多肽。通过使用包含2.6-kbp ORF部分的质粒进行杂交筛选分离出的mRNA确定了100-kDa多肽，抗糖蛋白II抗体和带状疱疹恢复期血清对该蛋白的免疫沉淀被纯化的糖蛋白II特异性阻断。我们得出结论，2.6-kbp ORF编码糖蛋白II。推测的糖蛋白II一级氨基酸序列与单纯疱疹病毒1型（HSV-1）gB的序列高度同源，这一结果与HSV和VZV基因组中各自基因的等效图谱位置以及最近报道的HSV-1 gB和VZV糖蛋白II的血清学交叉反应性一致。与gB的成熟基因产物不同，糖蛋白II的产物在还原凝胶中被描述为一对分子量约为60 kDa的糖蛋白，在非还原凝胶中是一种分子量约为125 - 140 kDa的单一糖蛋白的产物。测定了纯化的糖蛋白II的氨基末端序列并与推测的氨基酸序列进行比较。这种比较表明一级翻译产物在体内大约被切割成两半，并提示成熟的糖蛋白II是一种二硫键连接的异二聚体。
