Kjartansdóttir A, Lycke E, Norrby S R
Department of Infectious Diseases, Lund University, Sweden.
Arch Virol. 1996;141(12):2465-9. doi: 10.1007/BF01718644.
B-cell epitopes of varicella-zoster virus glycoprotein II were mapped by means of solid phase ELISA, synthetic oligopeptides (constructed according to the Davison-Scott sequencing of the varicella-zoster virus genome) and sera from varicellae and herpes zoster patients. The individual pattern of antibody peptide binding varied considerably but at least 9 more reactive sites seemed discernible. A 31-mer-peptide corresponding to a hydrophilic segment of the glycoprotein (aa 417-447) was constructed. This peptide reacted with 2 out of 4 varicellae and 5 out of 9 zoster sera, respectively.
通过固相酶联免疫吸附测定法、合成寡肽（根据水痘-带状疱疹病毒基因组的戴维森-斯科特测序构建）以及水痘和带状疱疹患者的血清，对水痘-带状疱疹病毒糖蛋白II的B细胞表位进行了定位。抗体与肽结合的个体模式差异很大，但至少有9个以上的反应位点似乎是可辨别的。构建了一个与该糖蛋白亲水片段（氨基酸417 - 447）相对应的31聚体肽。该肽分别与4份水痘血清中的2份以及9份带状疱疹血清中的5份发生反应。
