Petter C, Bourbon J, Maltier J P, Jost A
Teratology. 1977 Apr;15(2):149-57. doi: 10.1002/tera.1420150204.
Hematological and histological studies of the rabbit brachydactyly mutant were made to clarify the question of the blood abnormality that had previously been implicated in the teratogenic process leading to congenital amputation. The hemoglobin of fetal and adult br/br rabbits, studied by electrophoresis and cyanmethemoglobin assay, exhibited no differences from that of controls. The br/br fetuses were polycythemic and severely macrocytic. Fetal limb vessels showed thrombosis leading to hemorrhages in the extremities. The hepatic tissue was abnormal, being particularly poor in hematopoietic cells; blood cell macrocytosis was attributed to impaired erthropoiesis. Treatment of pregnant rabbits with folic acid plus vitamin B12 or with folinic acid was able simultaneously to prevent the fetal blood cell macrocytosis and the congenital abnormalities.
对兔短指畸形突变体进行了血液学和组织学研究，以阐明先前被认为与导致先天性截肢的致畸过程有关的血液异常问题。通过电泳和氰化高铁血红蛋白测定法研究了胎儿和成年br/br兔的血红蛋白，结果显示与对照组无差异。br/br胎儿出现红细胞增多症和严重的大红细胞症。胎儿肢体血管出现血栓形成，导致四肢出血。肝组织异常，造血细胞尤其稀少；血细胞大红细胞症归因于红细胞生成受损。用叶酸加维生素B12或亚叶酸治疗怀孕母兔能够同时预防胎儿血细胞大红细胞症和先天性异常。
