Gregory Holly N, Johnson Louis D V, Phillips James B
Department of Pharmacology, UCL School of Pharmacy, London, United Kingdom.
UCL Centre for Nerve Engineering, London, United Kingdom.
Front Cell Dev Biol. 2025 Apr 16;13:1567654. doi: 10.3389/fcell.2025.1567654. eCollection 2025.
Damage to peripheral nerves is common in major trauma cases, and current options for surgical repair are often not sufficient to promote satisfactory recovery of sensory and motor function. In this study we describe the development of a biomaterial scaffold with aligned nanofibrous topography and encapsulated neurotrophic factor, designed to direct and enhance axonal regeneration and so effectuate faster return of function.
Glial cell line-derived neurotrophic factor (GDNF) was loaded into aligned polycaprolactone (PCL) nanofibres using emulsion electrospinning, and the biomaterial was characterised alongside random and aligned PCL scaffolds without growth factor.
This fabrication route produced fine and uniform nanofibres with sustained release of GDNF over at least four weeks, and the aligned topography was able to orientate the growth of Schwann cells. Finally, the GDNF-loaded aligned nanofibrous scaffold significantly enhanced and directed the outgrowth of primary rat neurons cultured on its surface, demonstrating its promise as a pro-regenerative biomaterial for the surgical repair of nerve injury.
在重大创伤病例中，周围神经损伤很常见，而目前的手术修复方法往往不足以促进感觉和运动功能的满意恢复。在本研究中，我们描述了一种具有排列纳米纤维形貌并封装了神经营养因子的生物材料支架的研发，其旨在引导和增强轴突再生，从而实现功能更快恢复。
使用乳液静电纺丝将胶质细胞源性神经营养因子（GDNF）加载到排列的聚己内酯（PCL）纳米纤维中，并对该生物材料以及不含生长因子的随机和排列的PCL支架进行表征。
这种制造方法产生了精细且均匀的纳米纤维，GDNF能持续释放至少四周，且排列的形貌能够引导雪旺细胞的生长。最后，负载GDNF的排列纳米纤维支架显著增强并引导了培养在其表面的原代大鼠神经元的生长，证明了其作为用于神经损伤手术修复的促再生生物材料的潜力。
