Post-Graduating Program in Health and Environment, Tiradentes University, Av. Murilo Dantas, 300, Aracaju Sergipe 49010-390, Brazil.
Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil.
Curr Issues Mol Biol. 2021 Jun 11;43(1):335-352. doi: 10.3390/cimb43010028.
Plant extracts rich in phenolic compounds have been demonstrated to accelerate wound healing, but their use by oral route has been poorly studied. The leaves of are rich in phenolic acids and flavonoids. The goal of this study was to assess the healing properties of the oral administration of hydroalcoholic extract of leaves (HEVL) in a murine model. HEVL was obtained by Soxhlet and dynamic maceration, and their yield and phenolic acids and flavonoid contents were determined. For the wound healing assay, 8 mm wounds were performed on the back of 48 Wistar rats, assigned into four groups ( = 12): CTR (distilled water), HEVL100, HEVL200, and HEVL300 (HEVL at 100, 200, and 300 mg/kg, respectively). On days 7 and 14, wound closure rates were assessed, and the healing wounds were subjected to histological analysis. Soxhlet-obtained extract was selected for the wound healing assay because it provided a higher yield and phenolic acid and flavonoid contents. HEVL significantly reduced leukocytosis in the peripheral blood ( < 0.05), accelerated wound closure ( < 0.05), and improved collagenization ( < 0.05) on day 7, as well as enhanced the epidermal tissue thickness ( < 0.001) and elastic fiber deposition on day 14 ( < 0.01). Furthermore, HEVL promoted an increase in the histological grading of wound healing on both days 7 and 14 ( < 0.01). The doses of 200 and 300 mg/kg provided better results than 100 mg/Kg. Our data provide histological evidence that the oral administration of HEVL improves wound healing in rodents. Therefore, the extract can be a potential oral medicine for healing purposes.
植物提取物富含酚类化合物，已被证明可加速伤口愈合，但它们经口服途径的应用研究甚少。 叶片富含酚酸和类黄酮。本研究的目的是评估口服 水醇提取物（HEVL）在小鼠模型中的愈合特性。HEVL 通过索氏提取和动态浸提获得，并测定其产率和酚酸和类黄酮含量。为了进行伤口愈合试验，在 48 只 Wistar 大鼠的背部进行 8mm 伤口，分为四组（每组 12 只）：对照组（蒸馏水）、HEVL100、HEVL200 和 HEVL300（分别给予 HEVL 100、200 和 300mg/kg）。在第 7 天和第 14 天，评估伤口闭合率，并对愈合伤口进行组织学分析。选择索氏提取得到的提取物进行伤口愈合试验，因为它提供了更高的产率和酚酸和类黄酮含量。HEVL 显著降低外周血白细胞增多（<0.05），加速伤口闭合（<0.05），并在第 7 天改善胶原化（<0.05），以及在第 14 天增加表皮组织厚度（<0.001）和弹性纤维沉积（<0.01）。此外，HEVL 促进了第 7 天和第 14 天伤口愈合的组织学分级增加（<0.01）。200mg/kg 和 300mg/kg 的剂量比 100mg/kg 提供了更好的效果。我们的数据提供了组织学证据，表明口服 HEVL 可改善啮齿动物的伤口愈合。因此，该提取物可以是一种有潜力的用于愈合目的的口服药物。
