Department of Medicine, Carbone Cancer Center, University of Wisconsin-Madison, 1111 Highland Avenue, Madison, WI 53705, USA.
Lab Chip. 2018 Sep 26;18(19):3011-3017. doi: 10.1039/c8lc00431e.
Micromilling is an underutilized technique for fabricating microfluidic platforms that is well-suited for the diverse needs of the biologic community. This technique, however, produces culture surfaces that are considerably rougher than in commercially available culture platforms and the hydrophilicity of these surfaces can vary considerably depending on the choice of material. In this study, we evaluated the impact of surface topography and hydrophilicity in milled microfluidic devices on the cellular phenotype and function of primary human macrophages. We found that the rough culture surface within micromilled systems affected the phenotype of macrophages cultured in these devices. However, the presence, type, and magnitude of this effect was dependent on the surface hydrophilicity as well as exposure to chemical polarization signals. These findings confirm that while milled microfluidic systems are an effective platform for culture and analysis of primary macrophages, the topography and hydrophilicity of the culture surface within these systems should be considered in the planning and analysis of any macrophage experiments in which phenotype is relevant.
微铣削是一种未被充分利用的制造微流控平台的技术，非常适合生物界的各种需求。然而，这种技术所产生的培养表面比商业上可用的培养平台要粗糙得多，而且这些表面的亲水性会根据材料的选择而有很大的不同。在这项研究中，我们评估了微铣削微流控设备中表面形貌和润湿性对原代人巨噬细胞的细胞表型和功能的影响。我们发现，微铣削系统中的粗糙培养表面会影响在这些设备中培养的巨噬细胞的表型。然而，这种影响的存在、类型和程度取决于表面的润湿性以及是否接触化学极化信号。这些发现证实，虽然微铣削微流控系统是培养和分析原代巨噬细胞的有效平台，但在任何与表型相关的巨噬细胞实验的规划和分析中，都应考虑到这些系统中培养表面的形貌和润湿性。
