可调谐等离子体比色法，具有对外源 DNA 定量检测的反灵敏度。
Tunable plasmonic colorimetric assay with inverse sensitivity for extracellular DNA quantification.
机构信息
Biophysics Group, Department of Physics and Astronomy, University College London, Gower Street, London W1C 6BT, UK.
出版信息
Chem Commun (Camb). 2018 Oct 4;54(80):11260-11263. doi: 10.1039/c8cc05465g.
Extracellular DNA (eDNA) is a biomolecule commonly used to characterize microorganism communities in soil and aqueous environments. In this work we developed a gold nanorod (AuNR)-based colorimetric assay with inverse sensitivity and tunable dynamic range for eDNA. The effects of three key parameters, such as AuNR aspect ratio, DNA length and structure, have been identified allowing the assay to reach the detection levels necessary for the quantification of environmental eDNA.
细胞外 DNA（eDNA）是一种生物分子，常用于表征土壤和水生态环境中的微生物群落。在这项工作中，我们开发了一种基于金纳米棒（AuNR）的具有反向灵敏度和可调动态范围的比色测定法，用于检测 eDNA。确定了三个关键参数的影响，例如 AuNR 的纵横比、DNA 的长度和结构，使该测定法达到了定量环境 eDNA 所需的检测水平。
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