Department of Stomatology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China.
Oncol Rep. 2018 Nov;40(5):2798-2805. doi: 10.3892/or.2018.6663. Epub 2018 Aug 21.
MicroRNA‑21 (miR‑21) has been identified as an oncogene and confirmed to serve an important role in carcinogenesis in various types of cancer. However, the effect and mechanism of miR‑21 in oral squamous cell carcinoma (OSCC) has not been fully elucidated. In the present study, miR‑21 inhibitor and empty vector were transfected into OSCC cells, and non‑transfected cells were used as a blank control. The results indicated that when compared with the control and scramble groups, miR‑21 inhibitor suppressed the expression of miR‑21. Conversely, phosphatase and tensin homolog deletion on chromosome 10 (PTEN) was markedly upregulated, and a dual luciferase reporter assay revealed PTEN to be a target gene of miR‑21. Furthermore, miR‑21 inhibitor decreased the proliferation and invasion and enhanced the apoptosis of OSCC cells. There was no significant difference in cell proliferation, invasion and apoptosis between the control and scramble groups. The present data suggested that there may be a regulatory loop between miR‑21 and PTEN, and that miR‑21 inhibition affected the proliferative, invasive and apoptotic abilities of OSCC cells. These findings indicate that miR‑21 may be a possible novel target in the treatment of OSCC.
微小 RNA-21 (miR-21) 已被鉴定为一种癌基因，并被证实其在多种类型的癌症的发生中发挥重要作用。然而，miR-21 在口腔鳞状细胞癌 (OSCC) 中的作用和机制尚未完全阐明。在本研究中，将 miR-21 抑制剂和空载体转染至 OSCC 细胞中，未转染的细胞作为空白对照。结果表明，与对照组和 scramble 组相比，miR-21 抑制剂抑制了 miR-21 的表达。相反，磷酸酶和张力蛋白同源物缺失于染色体 10 (PTEN) 明显上调，并且双荧光素酶报告基因实验表明 PTEN 是 miR-21 的靶基因。此外，miR-21 抑制剂降低了 OSCC 细胞的增殖和侵袭能力，并增强了细胞凋亡。对照组和 scramble 组之间的细胞增殖、侵袭和凋亡无明显差异。这些数据表明，miR-21 和 PTEN 之间可能存在一个调控环，并且 miR-21 抑制影响 OSCC 细胞的增殖、侵袭和凋亡能力。这些发现表明，miR-21 可能是治疗 OSCC 的一个新的潜在靶点。
