黄曲霉毒素对大鼠肝脏线粒体细胞色素氧化酶活性的抑制作用。

Aflatoxin inhibition of rat liver mitochondrial cytochrome oxidase activity.

作者信息

Obidoa O

出版信息

Biochem Med Metab Biol. 1986 Jun;35(3):302-7. doi: 10.1016/0885-4505(86)90087-3.

Abstract

Aflatoxins B1, B2, G1, G2, and M1 have been evaluated for activity toward cytochrome oxidase in isolated rat liver mitochondria employing ferrocytochrome c and p-phenylene diamine as reductants. The aflatoxins inhibited the cytochrome oxidase activity to a greater extent when monitored by O2 uptake measurements than by substrate oxidation. AFG2 and AFM1 were the most potent (50-70%). Using oligomycin and 2,4-DNP as respiratory inhibitor and uncoupler, respectively, the aflatoxins appear to inhibit e- rather than energy transfer reactions. These toxins did not uncouple cytochrome oxidase activity.

摘要

已采用亚铁细胞色素c和对苯二胺作为还原剂，评估了黄曲霉毒素B1、B2、G1、G2和M1对分离的大鼠肝线粒体中细胞色素氧化酶的活性。与通过底物氧化监测相比，当通过氧气摄取测量进行监测时，黄曲霉毒素对细胞色素氧化酶活性的抑制程度更大。AFG2和AFM1的抑制作用最强（50%-70%）。分别使用寡霉素和2,4-二硝基苯酚作为呼吸抑制剂和解偶联剂，黄曲霉毒素似乎抑制电子传递而非能量传递反应。这些毒素并未使细胞色素氧化酶活性解偶联。