Silva Renata C A, Paranzini Cristiane S, Franco Leandro G, Miguel Marina P, Honsho Cristiane S, Souza Fabiana F
Hospital School of Veterinary, Universidade de Franca, UNIFRAN, Franca, Brazil.
School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista "Júlio de Mesquita Filho", UNESP, Botucatu, Brazil.
Reprod Domest Anim. 2018 Dec;53(6):1330-1338. doi: 10.1111/rda.13252. Epub 2018 Aug 22.
The aim of this study was to evaluate the effect of intratesticular injection of calcium chloride (CaCl ) combined with dimethyl sulphoxide (DMSO) as a chemical sterilization in dogs. Twelve dogs were divided into two groups: the treated group (n = 6), in which 15 mg/kg of a 7.5% CaCl solution combined with 0.5% DMSO was injected into each testicle (volume range 1.0-4.76 ml); and a control group (n = 6) that received the same volume/kg of 0.9% sodium chloride solution (NaCl). Semen characteristics pre- and post-treatment were evaluated. Serum testosterone concentration was determined before the injection (D-1) and at 15 (D15), 30 (D30) and 60 (D60) days after intratesticular injection. Testicle sizes and local pain were evaluated for 7 consecutive days (D1 to D7) and at D15, D30 and D60 after injection. At D60, testicle histological evaluation was performed after orchiectomy. No pain was observed by testicular palpation, with the exception of one dog in the treated group; this dog then received analgesic therapy. An increase in testicular volume was evident within 24 hr after treatment, followed by gradual reduction for 3 weeks. Five of 6 dogs from the treated group presented azoospermia at D15; the remaining dog presented at D30. There was no significant difference in testosterone concentrations in the treated group during the experimental period. Histological evaluation showed testicular degenerative lesions, especially at the proximal and middle portions. The results indicated that one injection of 7.5% CaCl combined with 0.5% DMSO into each testis is a viable alternative for canine sterilization.
本研究的目的是评估睾丸内注射氯化钙（CaCl）联合二甲基亚砜（DMSO）作为犬化学去势方法的效果。12只犬被分为两组：治疗组（n = 6），向每侧睾丸注射15 mg/kg的7.5% CaCl溶液与0.5% DMSO混合液（睾丸体积范围为1.0 - 4.76 ml）；对照组（n = 6），每千克体重注射相同体积的0.9%氯化钠溶液（NaCl）。评估治疗前后的精液特征。在睾丸内注射前（D - 1）以及注射后第15天（D15）、30天（D30）和60天（D60）测定血清睾酮浓度。连续7天（D1至D7）以及注射后D15、D30和D60评估睾丸大小和局部疼痛情况。在D60时，切除睾丸后进行组织学评估。除治疗组的1只犬外，触诊睾丸均未发现疼痛；该犬随后接受了镇痛治疗。治疗后24小时内睾丸体积明显增大，随后3周逐渐缩小。治疗组6只犬中有5只在D15时出现无精子症；其余1只在D30时出现。实验期间治疗组的睾酮浓度无显著差异。组织学评估显示睾丸有退行性病变，尤其是近端和中部。结果表明，每侧睾丸注射一次7.5% CaCl与0.5% DMSO混合液是犬去势的一种可行替代方法。
