Division of Molecular Medicine, Bose Institute, Centenary Campus, P 1/12, CIT Scheme, VIIM, Kolkata 700 054, India.
BMC Vet Res. 2011 Jul 21;7:39. doi: 10.1186/1746-6148-7-39.
Calcium chloride solution is an established injectable sterilant in dogs and other mammals. With cat populations a continuing problem, we sought to explore its first use in cats. Six cats per group were injected with 5%, 10% or 20% calcium chloride dihydrate in saline solution with lignocaine hydrochloride, a local anaesthetic.
At the 60th day post-injection, cat testes were collected and showed complete testicular necrosis and replacement by fibrous tissue; very low sperm counts; and reduction of serum testosterone by at least 70% in 20% dose. Androgenic enzyme activities and their expressions were also reduced in all the treated groups along with intra-testicular testosterone concentration was also low. Increased testicular lipid peroxidation, with reduced antioxidants and mitochondrial membrane potential, were evident following calcium chloride treatments. However, there were no apparent changes in serum concentrations of cortisol, fasting blood sugar level, blood urea nitrogen, packed cell volume, or total serum protein following calcium chloride injection, suggesting that this method of sterilization is not associated with any general stress response.
Calcium chloride solution demonstrates potential for androgenesis-eliminating nonsurgical sterilization of male cats in addition to its proven efficacy in dogs and other mammals.
氯化钙溶液是一种已被证实的犬类和其他哺乳动物的可注射灭菌剂。随着猫群问题的持续存在，我们试图探索其在猫身上的首次应用。每组 6 只猫分别注射 5%、10%或 20%的氯化钙二水合物和盐酸利多卡因生理盐水溶液，盐酸利多卡因是一种局部麻醉剂。
在注射后的第 60 天，收集猫的睾丸，结果显示完全的睾丸坏死和纤维组织取代；精子计数极低；20%剂量的血清睾酮至少减少了 70%。所有治疗组的雄激素酶活性及其表达也降低，同时睾丸内的睾酮浓度也较低。钙氯化物处理后，睾丸内的脂质过氧化增加，抗氧化剂和线粒体膜电位减少。然而，钙氯化物注射后，血清皮质醇浓度、空腹血糖水平、血尿素氮、红细胞压积或总血清蛋白均无明显变化，表明这种绝育方法与一般应激反应无关。
氯化钙溶液除了在犬类和其他哺乳动物中已被证实的有效性外，还显示出具有消除雄性猫雄激素的非手术绝育潜力。
