Wang Fu-cheng, Gong Dao-feng, Shi Wei-jing, Cheng Lei, Ji Dong-han
Zhong Yao Cai. 2016 May;39(5):1086-9.
To establish a method of simultaneous determination of chlorogenic acid,galuteolin,quercetin and acacetin in Chrysanthemi Flos by quantitative analysis of multi-components by single marker( QAMS),and determine the application value of QAMS in the quality control of Chrysanthemi Flos.
The analysis was established to use the Shim-pack C18( 250 mm × 4. 6 mm,5 μm),the mobile phase of water contained acetonitrile( A)-0. 1% phosphoric acid aqueous solution( B) in a gradient elution manner,the flow rate was 1. 0 m L / min,the column temperature was 30 ℃,the injection volume was 10 μL,the detection wavelength was set at348 nm. A characteristic spectrum was used for identification of four components. By using chlorogenic acid as reference,the relative corrective factors( RCF) of the other three components with reference to chlorogenic acid were calculated. The method was evaluated by comparison of the quantitative results through external standard method and QAMS method.
The RCF of galuteolin, quercetin and acacetin with reference to chlorogenic acid were 0. 6865,0. 9976 and 0. 6665,and the RSD( n = 6) were 0. 1439%,0. 2512%,0. 3971%,respectively. The results from QAMS method were not significantly different from those from external standard method.
The method with a single marker is accurate and feasible to evaluate the quality of galuteolin,quercetin and acacetin as reference to chlorogenic acid in Chrysanthemi Flos by QAMS.
建立一种采用一测多评法（QAMS）同时测定菊花中绿原酸、木犀草素、槲皮素和刺槐素的方法，并确定QAMS在菊花质量控制中的应用价值。
采用Shim-pack C18（250 mm×4.6 mm，5μm）色谱柱，以乙腈（A）-0.1%磷酸水溶液（B）为流动相进行梯度洗脱，流速为1.0 mL/min，柱温为30℃，进样量为10μL，检测波长设定为348 nm。采用特征图谱对4种成分进行鉴别。以绿原酸为参照，计算其他3种成分相对于绿原酸的相对校正因子（RCF）。通过外标法和QAMS法定量结果的比较对该方法进行评价。
木犀草素、槲皮素和刺槐素相对于绿原酸的RCF分别为0.6865、0.9976和0.6665，RSD（n=6）分别为0.1439%、0.2512%、0.3971%。QAMS法与外标法的结果无显著差异。
以绿原酸为参照，采用一测多评法评价菊花中木犀草素、槲皮素和刺槐素的质量，该方法准确可行。
