Song Xiao, Zhao Peng, Duan Xi, Wang Chang-li, Deng Han-shuang
Zhong Yao Cai. 2016 May;39(5):1110-3.
To screen the optimum method for deproteination of Dioscorea nipponica polysaccharides.
The ratio of protein removing and polysaccharides remaining were used as indicator,four deproteination methods were evaluated.
PapainSevage method was the best one, in which the deproteination rate was 91. 24%,and 80. 12% of polysaccharide was remained. The optimum conditions for deproteinization were as follows, hydrolysing the substrates with 2. 0% papain( p H 7. 0) at 55 ℃ for 122 min, and one times with Sevage method.
The papain-Sevage is the best deproteination method for purifying Dioscorea nipponica polysaccharides.
筛选日本薯蓣多糖脱蛋白的最佳方法。
以蛋白质去除率和多糖保留率为指标，对四种脱蛋白方法进行评价。
木瓜蛋白酶- Sevage法最佳，其脱蛋白率为91.24%，多糖保留率为80.12%。脱蛋白的最佳条件如下：用2.0%木瓜蛋白酶（pH 7.0）在55℃水解底物122分钟，再用Sevage法处理一次。
木瓜蛋白酶- Sevage法是纯化日本薯蓣多糖的最佳脱蛋白方法。
