Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Ultrason Sonochem. 2023 Aug;98:106520. doi: 10.1016/j.ultsonch.2023.106520. Epub 2023 Jul 8.
The crude Solanum tuberdsm polysaccharides (STP) were extracted with hot water. In the process of extraction, proteins, pigments, small molecules and salts in the mixture were removed by Sevage reagent, diatomite and distilled water dialysis, respectively. In addition, the process conditions of protein removal by response surface methodology (RSM) were optimized, and the optimum process conditions of Sevage method were established as follows: ultrasound power 350 W, ultrasound time 20 min, deproteinization twice, volume ratio of polysaccharide solution to Sevage reagent 1:1 (mL/mL). Under these conditions, the protein removal rate was 93.14%.
粗提的马铃薯多糖（STP）采用热水浸提法提取，在提取过程中分别用 Sevage 试剂、硅藻土和蒸馏水透析去除混合物中的蛋白质、色素、小分子和盐。此外，还采用响应面法（RSM）优化了去除蛋白质的工艺条件，确定了 Sevage 法的最佳工艺条件为：超声功率 350 W，超声时间 20 min，脱蛋白 2 次，多糖溶液与 Sevage 试剂的体积比为 1:1（mL/mL）。在此条件下，蛋白质去除率为 93.14%。
