State Key Laboratory of Tea Plant Biochemistry and Utilization , Anhui Agricultural University , Hefei , Anhui 230036 , P. R. China.
Key Laboratory of Agricultural Products Processing Engineering of Anhui Province , Anhui Agricultural University , Hefei , Anhui 230036 , P. R. China.
J Agric Food Chem. 2018 Sep 12;66(36):9565-9572. doi: 10.1021/acs.jafc.8b03067. Epub 2018 Aug 31.
It is well-known that anaerobic treatment has been considered as a utility process to accumulate γ-aminobutyric acid (GABA) in tea leaves. In this article, the nonvolatile differential compounds in picked-tea leaves between filled-N treatment and filled-CO treatment were compared in metabolic profiles and dynamic changes via ultrahigh performance liquid chromatography linked to a hybrid quadrupole orthogonal time-of-flight mass spectrometer (UPLC-Q-TOF-MS). Multivariate analysis and heat map of hierarchical clustering analysis indicated that filled-N treatment resulted in a wider range of metabolic perturbation than filled-CO treatment, but GABA accumulates faster and more significantly under filled-CO treatment than other treatment. The differential metabolites in anaerobic treatment were mainly reflected in the levels of glucose metabolism and amino acid metabolism, and the main differential pathway included the glyoxylate metabolism pathway, galactose metabolism, and phenylalanine metabolism. These metabolomic analyses were also evaluated to illuminate the physiological adaptive strategies of tea adopted to tolerate certain anaerobic stress types.
众所周知，厌氧处理已被认为是在茶叶中积累γ-氨基丁酸（GABA）的有效方法。在本文中，通过超高效液相色谱-串联四极杆正交飞行时间质谱联用（UPLC-Q-TOF-MS），比较了填氮和填二氧化碳处理的采摘后茶叶之间的非挥发性差异化合物在代谢谱和动态变化方面的差异。多变量分析和层次聚类热图分析表明，填氮处理比填二氧化碳处理引起的代谢干扰范围更广，但与其他处理相比，填二氧化碳处理能更快、更显著地积累 GABA。厌氧处理的差异代谢物主要反映在葡萄糖代谢和氨基酸代谢水平上，主要差异途径包括乙醛酸代谢途径、半乳糖代谢途径和苯丙氨酸代谢途径。这些代谢组学分析也被用来阐明茶叶为了耐受特定的厌氧胁迫类型而采用的生理适应策略。
