Yan Ge, Shi Yujie, Chen Fangfang, Mu Chunsheng, Wang Junfeng
Key Laboratory of Vegetation Ecology of the Ministry of Education, Institute of Grassland Science, Jilin Songnen Grassland Ecosystem National Observation and Research Station, Northeast Normal University, Changchun 130024, China.
Plants (Basel). 2022 Jun 2;11(11):1494. doi: 10.3390/plants11111494.
To elucidate the physiological and metabolic mechanism of perennial grass responses to alkali stress, we selected (), a salt-tolerant perennial rhizomatous species of the family Poaceae as experimental material. We conducted a pot experiment in a greenhouse and measured the biomass, physiological characteristics, metabonomic, and corresponding metabolites. Our results showed that alkali stress significantly inhibited seedling growth and photosynthesis, which caused ion imbalance and carbon deficiency, but the alkali stress significantly increased the nitrogen and ATP contents. The metabolic analysis indicated that alkali stress markedly enhanced the contents of nucleotides, amino acids, and organic acids, but it decreased soluble sugar contents. Pathway enrichment analysis showed that the glutamine synthetase/glutamate synthase () cycle, which was related to nitrogen metabolism, was most significantly affected by alkali stress. The contents of glutamine synthetase (GS) and glutamate synthetase (GOGAT) involved in this pathway were also significantly increased. Our results not only verified the important roles of some amino acids and organic acids in resisting alkali stress, but also further proved that nucleotides and the cycle related to nitrogen metabolism played critical roles for seedlings in response to alkali stress.
为阐明多年生草本植物对碱胁迫的生理和代谢机制，我们选取了禾本科一种耐盐多年生根茎型物种（）作为实验材料。我们在温室中进行了盆栽试验，并测定了生物量、生理特性、代谢组学及相应代谢物。结果表明，碱胁迫显著抑制幼苗生长和光合作用，导致离子失衡和碳缺乏，但碱胁迫显著增加了氮和ATP含量。代谢分析表明，碱胁迫显著提高了核苷酸、氨基酸和有机酸的含量，但降低了可溶性糖含量。通路富集分析表明，与氮代谢相关的谷氨酰胺合成酶/谷氨酸合酶（）循环受碱胁迫影响最为显著。参与该通路的谷氨酰胺合成酶（GS）和谷氨酸合酶（GOGAT）的含量也显著增加。我们的结果不仅验证了一些氨基酸和有机酸在抵抗碱胁迫中的重要作用，还进一步证明了核苷酸以及与氮代谢相关的循环对幼苗响应碱胁迫起着关键作用。
