McCaslin P P, Morgan W W
Brain Res. 1986 May 28;374(2):367-70. doi: 10.1016/0006-8993(86)90431-2.
Ovariectomized female Sprague-Dawley rats were trained to swim a 2.5 m course. On the day of the experiment the time required for each animal to swim the course was determined, and then the animal received either saline or one of 4 dosages of sodium pentobarbital, intravenously. Four minutes after treatment the animal's swim time was again recorded, and the animal was immediately killed by microwave irradiation. The cerebellum was collected for subsequent determination of cyclic guanosine monophosphate (cGMP). Pentobarbital caused a dosage-dependent suppression of cerebellar cGMP which was highly correlated with a drug-induced increase in the time required to swim 2.5 m. These data provide the first evidence of a correlation between a barbiturate-induced effect on a neurochemical parameter and impaired performance of a learned behavior.
将雌性Sprague-Dawley大鼠去卵巢后，训练它们游完2.5米的路程。在实验当天，测定每只动物游完该路程所需的时间，然后给动物静脉注射生理盐水或4种剂量戊巴比妥钠中的一种。给药4分钟后，再次记录动物的游泳时间，并立即通过微波照射将动物处死。收集小脑用于随后测定环磷酸鸟苷（cGMP）。戊巴比妥钠引起小脑cGMP的剂量依赖性抑制，这与药物诱导的游完2.5米所需时间增加高度相关。这些数据首次证明了巴比妥酸盐对神经化学参数的影响与学习行为表现受损之间的相关性。
