大鼠骨骼肌中钙蛋白酶I的免疫金电子显微镜定位
Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats.
作者信息
Yoshimura N, Murachi T, Heath R, Kay J, Jasani B, Newman G R
出版信息
Cell Tissue Res. 1986;244(2):265-70. doi: 10.1007/BF00219201.
PMID:3013409
Abstract
By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.
摘要
通过使用双亲和纯化的一抗以及胶体金偶联的二抗，结果表明钙蛋白酶I（一种由微摩尔浓度的Ca2+激活的半胱氨酸蛋白酶）在大鼠趾长伸肌（EDL）的I带区域中主要位于细胞内，但并非仅与Z线相关。
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