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大鼠骨骼肌中钙蛋白酶I的免疫金电子显微镜定位 - Suppr | 超能文献

大鼠骨骼肌中钙蛋白酶I的免疫金电子显微镜定位

Immunogold electron-microscopic localisation of calpain I in skeletal muscle of rats.

作者信息

Yoshimura N, Murachi T, Heath R, Kay J, Jasani B, Newman G R

出版信息

Cell Tissue Res. 1986;244(2):265-70. doi: 10.1007/BF00219201.

DOI:10.1007/BF00219201

Abstract

By using a double-affinity-purified first antibody and colloidal gold-conjugated second antibody, it is shown that calpain I (a cysteine proteinase activated by micromolar concentrations of Ca2+) has a predominant intracellular location in the I-band region of the extensor digitorum longus (EDL) muscle of the rat, but is not exclusively associated with the Z-line.

摘要

通过使用双亲和纯化的一抗以及胶体金偶联的二抗，结果表明钙蛋白酶I（一种由微摩尔浓度的Ca2+激活的半胱氨酸蛋白酶）在大鼠趾长伸肌（EDL）的I带区域中主要位于细胞内，但并非仅与Z线相关。

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J Biol Chem. 1964 Jan;239:149-55.

2

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J Biochem. 1980 Jan;87(1):343-6. doi: 10.1093/oxfordjournals.jbchem.a132743.

3

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Eur J Histochem. 2013 Jan 14;57(1):e2. doi: 10.4081/ejh.2013.e2.

4

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