Ishiura S, Sugita H, Nonaka I, Imahori K
J Biochem. 1980 Jan;87(1):343-6. doi: 10.1093/oxfordjournals.jbchem.a132743.
Calcium-activated neutral protease (CANP) was demonstrated in myofibrils, especially at the Z-band in chicken glycerinated myofibrils, by an immunofluorescent method. The amount of CANP bound to myofibrils was approximately 4 percent of that contained in the whole muscle homogenate. No immunological difference was recognized between the myofibril-bound CANP and the soluble one when examined by Ouchterlony's immunodiffusion analysis. A possible role of myofilbril-bound CANP in the physiological turnover of the myofibrillar proteins is discussed.
