Hewlins M J, Weeks I, Jasani B
J Immunol Methods. 1984 May 11;70(1):111-8. doi: 10.1016/0022-1759(84)90395-8.
Methyl 3-(2,4- dinitrophenylamino ) propionimidate hydrochloride (DNP-N-IE), methyl 3-(2,4-dinitrophenyl-N-methylamino) propionimidate hydrochloride (DNP- NMe -IE), and methyl 3-(2,4- dinitrophenylthio ) propionimidate hydrochloride (DNP-S-IE) have been prepared. DNP-N-IE and DNP- NMe -IE both react efficiently at 0-2 degrees C and pH 7-9.5 with sheep immunoglobulin G. At least 12 DNP groups can be introduced into the antibody protein without causing any significant change in its antigen binding affinity or capacity.
已制备出盐酸3-(2,4-二硝基苯氨基)丙亚氨酸甲酯(DNP-N-IE)、盐酸3-(2,4-二硝基-N-甲基苯氨基)丙亚氨酸甲酯(DNP-NMe-IE)和盐酸3-(2,4-二硝基苯硫基)丙亚氨酸甲酯(DNP-S-IE)。DNP-N-IE和DNP-NMe-IE在0至2摄氏度及pH值7至9.5的条件下均能与绵羊免疫球蛋白G高效反应。可将至少12个DNP基团引入抗体蛋白，而不会使其抗原结合亲和力或能力发生任何显著变化。
