Ziganshina Elvira E, Mohammed Waleed S, Shagimardanova Elena I, Shigapova Leyla H, Ziganshin Ayrat M
Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kremlyovskaya str. 18, Kazan, 420008, Russia.
Laboratory of Extreme Biology, Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420021, Russia.
BMC Res Notes. 2018 Aug 22;11(1):606. doi: 10.1186/s13104-018-3710-1.
Bacillus species, belonging to the family Bacillaceae, are rod-shaped aerobic or facultative anaerobic Gram-positive bacteria that can be isolated from various environmental niches. Bacillus pumilus strains are resistant to unfavorable conditions such as UV, HO and chemical disinfection. Furthermore, B. pumilus strains synthesize multifarious important enzymes and can be used in the production of some fermented foods, bioremediation of wastewater systems and biodegradation of environmental contaminants. Hence, investigation at the genomic level is required to understand their ecology, genetics and potential applications.
In this research, we provide the genomic insights into one Bacillus species (EZ-C07) isolated from digested agricultural waste materials. The draft genome of the strain EZ-C07 consists of 3,724,869 bp with 3890 coding sequences and 41.5% G + C content. Based on 16S rRNA gene sequence analysis followed by in silico DNA-DNA hybridization studies, the strain EZ-C07 was identified as Bacillus pumilus belonging to the family Bacillaceae within the phylum Firmicutes. The whole genome shotgun project of B. pumilus strain EZ-C07 can be accessed at DDBJ/ENA/GenBank under the Accession QLVI00000000.
芽孢杆菌属属于芽孢杆菌科，是杆状需氧或兼性厌氧革兰氏阳性菌，可从各种环境生态位中分离得到。短小芽孢杆菌菌株对紫外线、羟基自由基和化学消毒等不利条件具有抗性。此外，短小芽孢杆菌菌株能合成多种重要酶类，可用于某些发酵食品的生产、废水系统的生物修复以及环境污染物的生物降解。因此，需要在基因组水平上进行研究以了解它们的生态学、遗传学和潜在应用。
在本研究中，我们提供了对从消化后的农业废料中分离出的一种芽孢杆菌（EZ-C07）的基因组见解。菌株EZ-C07的基因组草图由3,724,869个碱基对组成，有3890个编码序列，G+C含量为41.5%。基于16S rRNA基因序列分析及随后的电子DNA-DNA杂交研究，菌株EZ-C07被鉴定为厚壁菌门芽孢杆菌科的短小芽孢杆菌。短小芽孢杆菌菌株EZ-C07的全基因组鸟枪法测序项目可在DDBJ/ENA/GenBank上获取，登录号为QLVI00000000。
