College of Food Science and Technology, Hebei Agricultural University, Baoding, China.
Microbiologyopen. 2019 Mar;8(3):e00663. doi: 10.1002/mbo3.663. Epub 2018 Aug 31.
Aflatoxin M (AFM ) is a potent mycotoxin which causes serious health concerns in developing countries, where it is mainly found in milk, meat, and other foods. Biological detoxification is a promising method for eliminating AFM . The aim of this work was to search for AFM -degrading bacterial strains from animal waste, soil, and activated sludge. High-performance liquid chromatography and Fourier-transform infrared spectroscopy were used to analyze the AFM degradation products. A strain designated E-1-1-1 was obtained from African elephants feces, with the degradation ratio of AFM reaching 89.55% in 12 hr. Based on morphology, physiological and biochemical tests, and 16S rRNA gene sequence analysis, strain E-1-1-1 was identified as Bacillus pumilus. The culture supernatant of B. pumilus E-1-1-1 degraded AFM effectively, whereas the cells and cell extracts of B. pumilus E-1-1-1 were far less effective. Carbon and nitrogen sources had highly significant effects on the degradation of AFM by B. pumilus E-1-1-1. The AFM -degrading strain, B. pumilus E1-1-1, could have great potential in industrial applications.
黄曲霉毒素 M（AFM ）是一种强毒性真菌毒素，主要存在于牛奶、肉类和其他食物中，对发展中国家的健康构成严重威胁。生物解毒是消除 AFM 的一种很有前途的方法。本工作旨在从动物粪便、土壤和活性污泥中寻找能够降解 AFM 的细菌菌株。采用高效液相色谱和傅里叶变换红外光谱分析 AFM 的降解产物。从非洲象粪便中获得一株编号为 E-1-1-1 的菌株，在 12 小时内对 AFM 的降解率达到 89.55%。根据形态学、生理生化试验和 16S rRNA 基因序列分析，菌株 E-1-1-1 被鉴定为短小芽孢杆菌。短小芽孢杆菌 E-1-1-1 的培养上清液能有效降解 AFM ，而短小芽孢杆菌 E-1-1-1 的细胞和细胞提取物则效果较差。碳源和氮源对短小芽孢杆菌 E-1-1-1 降解 AFM 有显著影响。AFM 降解菌株短小芽孢杆菌 E1-1-1 可能具有巨大的工业应用潜力。
