Department of Gynecology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, 471000, Henan, China.
Department of Gynecology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, 471000, Henan, China.
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2956-2962. doi: 10.1016/j.bbrc.2018.08.077. Epub 2018 Aug 19.
Long non-coding RNAs (lncRNAs) are a crucial member of non-coding RNA family, and increasing evidence demonstrates that lncRNAs participate in the initiation and progression of cancers. Our study aimed to explore the role of lncRNA TTN-AS1 in cervical cancer (CC) development. In the present study, our results showed that TTN-AS1 was substantially increased in CC tissues and cell lines, high expression of TTN-AS1 was correlated with advanced FIGO stage, poor differentiation, lymph node metastasis, and poor overall survival of CC patients. Function assays showed that TTN-AS1 inhibition decreased the proliferation and invasion of CC cells both in vitro and in vivo. Mechanistically, we revealed that TTN-AS1 could positively modulate E2F3 expression via sponging miR-573 in CC cells. Together, our study revealed that lncRNA TTN-AS1 was involved in the progression of CC cells by regulation of miR-573-E2F3 axis, which offered a new insight into the treatment strategies of CC.
长链非编码 RNA（lncRNAs）是非编码 RNA 家族的重要成员，越来越多的证据表明 lncRNAs 参与了癌症的发生和发展。本研究旨在探讨 lncRNA TTN-AS1 在宫颈癌（CC）发展中的作用。在本研究中，我们的结果表明 TTN-AS1 在 CC 组织和细胞系中显著增加，高表达的 TTN-AS1 与 FIGO 晚期、低分化、淋巴结转移和 CC 患者的总体生存率差相关。功能分析表明，TTN-AS1 抑制可降低 CC 细胞的增殖和侵袭，无论是在体外还是体内。机制上，我们揭示了 TTN-AS1 可通过在 CC 细胞中海绵吸附 miR-573 正向调节 E2F3 的表达。总之，我们的研究表明，lncRNA TTN-AS1 通过调节 miR-573-E2F3 轴参与 CC 细胞的进展，为 CC 的治疗策略提供了新的思路。
