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从小鼠骨骼肌肌动蛋白信使核糖核酸中分离并鉴定互补脱氧核糖核酸克隆

Isolation and characterization of cDNA clones from mouse skeletal muscle actin mRNA.

作者信息

Leader D P, Gall I, Campbell P, Frischauf A M

出版信息

DNA. 1986 Jun;5(3):235-8. doi: 10.1089/dna.1986.5.235.

DOI:10.1089/dna.1986.5.235
PMID:3013550
Abstract

The sequence corresponding to approximately 98% of mouse skeletal muscle actin mRNA was determined from cDNA clones isolated from a library of recombinants in pBR322. One of these clones contains DNA corresponding to the complete amino acid coding region and a large part of the 5' and 3' untranslated regions of the mRNA. Comparison of the mouse coding region (conserved at the amino acid level) and noncoding regions with the corresponding regions of the rat skeletal muscle actin gene indicates that the noncoding regions have also been under selective pressure during evolution.

摘要

从pBR322重组体文库中分离得到的cDNA克隆，测定了约98%的小鼠骨骼肌肌动蛋白mRNA对应的序列。其中一个克隆含有与mRNA完整氨基酸编码区以及5'和3'非翻译区大部分相对应的DNA。将小鼠编码区（在氨基酸水平保守）和非编码区与大鼠骨骼肌肌动蛋白基因的相应区域进行比较，结果表明非编码区在进化过程中也受到了选择压力。

相似文献

1
Isolation and characterization of cDNA clones from mouse skeletal muscle actin mRNA.从小鼠骨骼肌肌动蛋白信使核糖核酸中分离并鉴定互补脱氧核糖核酸克隆
DNA. 1986 Jun;5(3):235-8. doi: 10.1089/dna.1986.5.235.
2
Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.人骨骼肌α-肌动蛋白cDNA克隆的分离与鉴定
Nucleic Acids Res. 1983 Jun 11;11(11):3503-16. doi: 10.1093/nar/11.11.3503.
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The 3'-untranslated sequence of human skeletal muscle alpha-actin mRNA.人类骨骼肌α-肌动蛋白信使核糖核酸的3'非翻译序列。
J Muscle Res Cell Motil. 1984 Aug;5(4):457-64. doi: 10.1007/BF00818263.
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The structure of a cDNA clone corresponding to mouse cardiac muscle actin mRNA.与小鼠心肌肌动蛋白mRNA对应的cDNA克隆的结构。
Biosci Rep. 1986 Aug;6(8):741-7. doi: 10.1007/BF01116542.
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Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.人α-、β-和γ-肌动蛋白mRNA全长cDNA克隆的分离与鉴定：骨骼肌肌动蛋白而非细胞质肌动蛋白具有一个随后会被去除的氨基端半胱氨酸。
Mol Cell Biol. 1983 May;3(5):787-95. doi: 10.1128/mcb.3.5.787-795.1983.
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Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase.编码大鼠骨骼肌肌酸激酶的cDNA克隆的分离与序列分析。
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Expression of the genes coding for the skeletal muscle and cardiac actions in the heart.编码骨骼肌和心脏活动的基因在心脏中的表达。
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Conserved and unique sequences in the 3'-untranslated region of rat smooth-muscle alpha-actin mRNA.大鼠平滑肌α-肌动蛋白mRNA 3'非翻译区的保守序列和独特序列
Gene. 1988 Sep 30;69(2):345-8. doi: 10.1016/0378-1119(88)90445-3.
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Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.肌动蛋白mRNA非翻译区的进化保守性：人β-肌动蛋白cDNA的DNA序列
Nucleic Acids Res. 1984 Feb 10;12(3):1687-96. doi: 10.1093/nar/12.3.1687.
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Skeletal muscle actin mRNA. Characterization of the 3' untranslated region.骨骼肌肌动蛋白信使核糖核酸。3'非翻译区的特征
Nucleic Acids Res. 1981 Feb 11;9(3):579-89. doi: 10.1093/nar/9.3.579.

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