Leader D P, Gall I, Campbell P C
Biosci Rep. 1986 Aug;6(8):741-7. doi: 10.1007/BF01116542.
A cDNA library was constructed from mouse cardiac muscle mRNA, and a clone corresponding to part of the mRNA for the cardiac muscle isoform of actin was isolated from this library. The nucleotide sequence of the cloned insert was determined and was found to contain almost the complete amino acid coding region for actin (only codons for the first two amino acids, absent from the mature protein, were lacking) and a substantial portion derived from the 3' untranslated region of the mRNA. Comparison of the latter with the corresponding region in cardiac actin mRNA from man and rat showed that this 3' untranslated region has been subject to conservational pressure during evolution. However a comparison with the corresponding region in skeletal muscle actin mRNAs indicated that the pattern of conservation is quite different in the two striated muscle actin isoforms.
从小鼠心肌mRNA构建了一个cDNA文库，并从该文库中分离出一个与肌动蛋白心肌同工型mRNA部分相对应的克隆。测定了克隆插入片段的核苷酸序列，发现其包含肌动蛋白几乎完整的氨基酸编码区（仅缺少成熟蛋白中不存在的前两个氨基酸的密码子）以及来自mRNA 3'非翻译区的相当一部分。将后者与人及大鼠心肌肌动蛋白mRNA中的相应区域进行比较，结果表明该3'非翻译区在进化过程中受到了保守压力。然而，与骨骼肌肌动蛋白mRNA中的相应区域进行比较表明，在两种横纹肌肌动蛋白同工型中，保守模式有很大不同。
