Liu Zikou, Silke John, Hildebrand Joanne M
Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
Department of Medical Biology, University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2018;1857:53-61. doi: 10.1007/978-1-4939-8754-2_5.
Necroptosis is a caspase-independent form of programmed cell death that is induced by a variety of different signalling cascades-all culminating in the activation of the pseudokinase mixed lineage kinase domain-like (MLKL). TNF-induced necroptosis is the most intensively studied of these pathways. Here we describe reagents and cell-based techniques that can be used to investigate TNF-mediated necroptosis in the lab.
坏死性凋亡是一种不依赖半胱天冬酶的程序性细胞死亡形式，由多种不同的信号级联反应诱导——所有这些最终都导致假激酶混合谱系激酶结构域样蛋白（MLKL）的激活。肿瘤坏死因子（TNF）诱导的坏死性凋亡是这些途径中研究最深入的。在这里，我们描述了可用于在实验室研究TNF介导的坏死性凋亡的试剂和基于细胞的技术。
