Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, Netherlands.
Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia.
J Clin Endocrinol Metab. 2019 Jan 1;104(1):49-56. doi: 10.1210/jc.2018-00995.
Vitamin D deficiency in obesity has been linked to insulin resistance. However, studies that examined the association between plasma 25-hydroxyvitamin D3 [25(OH)D3] as well as plasma 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and tissue-specific insulin sensitivity are scarce. Furthermore, vitamin D receptor (VDR) and vitamin D-metabolizing enzymes [cytochrome 450 (CYP)] expression in adipose tissue (AT) might affect AT insulin sensitivity.
To investigate the association between body mass index (BMI) and plasma 25(OH)D3 and 1,25(OH)2D3, AT VDR; between plasma 25(OH)D3, 1,25(OH)2D3, AT VDR, and tissue-specific insulin sensitivity in individuals with overweight/obesity.
This analysis included 92 adult individuals (BMI, >25 kg/m2). A two-step hyperinsulinemic-euglycemic clamp with a [6,6-2H2]-glucose tracer was performed to assess tissue-specific insulin sensitivity. Abdominal subcutaneous AT (SAT) mRNA expression of VDR and CYP was determined by using quantitative RT-PCR.
University medical center.
Plasma 25(OH)D3, 1,25(OH)2D3, 1,25(OH)2D3/25(OH)D3 ratio, SAT VDR and CYPs mRNA, and tissue-specific insulin sensitivity.
BMI was inversely associated with plasma 25(OH)D3 (β = -0.274; P = 0.011) but not with plasma 1,25(OH)2D3. Plasma 25(OH)D3 was not related to CYPs or VDR expression in SAT. Plasma 1,25(OH)2D3 and 25(OH)D3 were not related to tissue-specific insulin sensitivity. Interestingly, SAT VDR mRNA was negatively associated with AT insulin sensitivity (β = -0.207; P = 0.025).
BMI was inversely associated with 25(OH)D3 concentrations, which could not be explained by alterations in SAT VDR and CYP enzymes. Plasma vitamin D metabolites were not related to tissue-specific insulin sensitivity. However, VDR expression in SAT was negatively associated with AT insulin sensitivity.
肥胖症患者的维生素 D 缺乏与胰岛素抵抗有关。然而，目前关于血浆 25-羟维生素 D3 [25(OH)D3]和血浆 1,25-二羟维生素 D3 [1,25(OH)2D3]与组织特异性胰岛素敏感性之间的关联的研究很少。此外，脂肪组织（AT）中的维生素 D 受体（VDR）和维生素 D 代谢酶（细胞色素 450（CYP））的表达可能会影响 AT 胰岛素敏感性。
研究超重/肥胖个体的体重指数（BMI）与血浆 25(OH)D3 和 1,25(OH)2D3、AT VDR 之间的关系；血浆 25(OH)D3、1,25(OH)2D3、AT VDR 与组织特异性胰岛素敏感性之间的关系。
本分析包括 92 名成年个体（BMI＞25kg/m2）。采用两步法高胰岛素正葡萄糖钳夹技术联合[6,6-2H2]-葡萄糖示踪剂评估组织特异性胰岛素敏感性。采用实时定量 RT-PCR 法测定腹部皮下脂肪组织（SAT）中 VDR 和 CYP 的 mRNA 表达。
大学医学中心。
血浆 25(OH)D3、1,25(OH)2D3、1,25(OH)2D3/25(OH)D3 比值、SAT VDR 和 CYPmRNA 及组织特异性胰岛素敏感性。
BMI 与血浆 25(OH)D3 呈负相关（β=-0.274；P=0.011），但与血浆 1,25(OH)2D3 无关。血浆 25(OH)D3 与 SAT 中的 CYP 或 VDR 表达无关。血浆 1,25(OH)2D3 和 25(OH)D3 与组织特异性胰岛素敏感性无关。有趣的是，SAT VDR mRNA 与 AT 胰岛素敏感性呈负相关（β=-0.207；P=0.025）。
BMI 与 25(OH)D3 浓度呈负相关，而这不能用 SAT VDR 和 CYP 酶的改变来解释。血浆维生素 D 代谢物与组织特异性胰岛素敏感性无关。然而，SAT 中的 VDR 表达与 AT 胰岛素敏感性呈负相关。
