Usama Nagla, El-Sayed Amr, Gamal Mohamed, Mekheimer Salma, Elhadidy Khaled, Awadein Mohamed, Farid Mahmoud
Medical Laboratory Technology Department, Faculty of Applied Health Science Technology, Misr University for Science and Technology, Cairo, Egypt.
Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt.
Diabetol Metab Syndr. 2024 Aug 13;16(1):195. doi: 10.1186/s13098-024-01427-4.
Vitamin D deficiency, insulin resistance, dyslipidemia, and APOE genotyping are implicated in the pathogenesis of obesity and type 2 diabetes mellitus (T2DM). We wanted to find out if there was a link between a lack of 25(OH) vitamin D, HOMA-IR, and lipids and APOE genotyping in obese people with and without T2DM.
We divided 300 Egyptians of both sexes into three groups in a case-control study: 100 obese cases with a body mass index of more than 30, 100 obese cases diagnosed with T2DM, and 100 controls with a body mass index of less than 30. Levels of 25 (OH) vitamin D, fasting blood sugar (FBS), HbA1C, fasting insulin, HOMA-IR, and lipid profile parameters were measured, and APOE genotypes were assessed using Applied BiosystemsTM TaqMan SNP Genotyping Assays.
Higher levels of FBS, fasting insulin, HOMA-IR, and dyslipidemia were found in obese people with and without T2DM compared to the control group (p < 0.05). Lower levels of 25(OH) vitamin D were also found. Insulin resistance and lipid profile parameters, particularly in obese cases with T2DM, inversely correlate with vitamin D deficiency. The APOE genotyping analysis revealed strong links between vitamin D levels and certain APOE genotypes. Independent of metabolic parameters, higher vitamin D levels were associated with lower odds of E3/E4 and E4/E4 genotypes among obese cases with T2DM.
This study highlights the independent role of vitamin D deficiency in modulating APOE genotypes in obese T2DM individuals. The findings suggest potential implications for personalized interventions targeting vitamin D status to mitigate genetic predispositions to metabolic disorders such as obesity and T2DM.
维生素D缺乏、胰岛素抵抗、血脂异常和载脂蛋白E（APOE）基因分型与肥胖症和2型糖尿病（T2DM）的发病机制有关。我们想弄清楚在患有和未患有T2DM的肥胖人群中，25（OH）维生素D缺乏、稳态模型评估胰岛素抵抗（HOMA-IR）、血脂与APOE基因分型之间是否存在联系。
在一项病例对照研究中，我们将300名埃及男女分为三组：100名体重指数超过30的肥胖病例，100名被诊断为T2DM的肥胖病例，以及100名体重指数低于30的对照组。测量了25（OH）维生素D、空腹血糖（FBS）、糖化血红蛋白（HbA1C）、空腹胰岛素、HOMA-IR和血脂参数水平，并使用应用生物系统公司的TaqMan单核苷酸多态性基因分型检测法评估APOE基因型。
与对照组相比，患有和未患有T2DM的肥胖人群中FBS、空腹胰岛素、HOMA-IR和血脂异常水平更高（p < 0.05）。还发现25（OH）维生素D水平较低。胰岛素抵抗和血脂参数，特别是在患有T2DM的肥胖病例中，与维生素D缺乏呈负相关。APOE基因分型分析揭示了维生素D水平与某些APOE基因型之间的紧密联系。在患有T2DM的肥胖病例中，独立于代谢参数，较高的维生素D水平与E3/E4和E4/E4基因型的较低几率相关。
本研究强调了维生素D缺乏在调节肥胖T2DM个体APOE基因型方面的独立作用。研究结果表明，针对维生素D状态进行个性化干预可能对减轻肥胖和T2DM等代谢紊乱的遗传易感性具有潜在意义。
