Ehresmann D W, Hogan R N
Intervirology. 1986;25(2):103-10. doi: 10.1159/000149663.
Coinfected mice were examined for a possible interaction between the scrapie agent and an adenovirus. A low titer (10(2) TCD50) of mouse adenovirus (MAdV) caused a significant acceleration of clinical signs of scrapie in mice infected 128 days previously with scrapie. In this experiment, the coinfected mice died 19 days earlier than mice infected with scrapie alone. When a higher titer of MAdV (10(4)-10(5) PFU) was used, a more drastic acceleration of scrapie disease was seen in mice infected 85 and 110 days previously with scrapie. At 85 days, coinfection caused mice to die 37 days earlier than mice infected with scrapie alone, whereas at 110 days, coinfection caused mice to die 52 days earlier than mice infected with scrapie alone. MAdV alone caused no clinical disease in normal mice. The brains of coinfected mice and mice that had been infected with scrapie alone showed a histopathology consistent with scrapie. A possible explanation for these findings is that the replication of the scrapie agent is accelerated by adenovirus. Defective parvoviruses are known to be helped by adenoviruses. Spleens from coinfected mice but not from mice infected with MAdV alone yielded, in cultures of BALB 3T3 cells, infectious MAdV and one or two smaller agents with the dimension and shape of a parvovirus.
对同时感染的小鼠进行检查，以探究羊瘙痒病病原体与腺病毒之间可能存在的相互作用。低滴度（10(2) TCD50）的小鼠腺病毒（MAdV）可使128天前感染羊瘙痒病的小鼠的羊瘙痒病临床症状显著加速。在该实验中，同时感染的小鼠比仅感染羊瘙痒病的小鼠早19天死亡。当使用更高滴度的MAdV（10(4)-10(5) PFU）时，在85天和110天前感染羊瘙痒病的小鼠中观察到羊瘙痒病病情更剧烈地加速。在85天时，同时感染使小鼠比仅感染羊瘙痒病的小鼠早37天死亡，而在110天时，同时感染使小鼠比仅感染羊瘙痒病的小鼠早52天死亡。单独的MAdV在正常小鼠中未引起临床疾病。同时感染的小鼠和仅感染羊瘙痒病的小鼠的大脑显示出与羊瘙痒病一致的组织病理学特征。对这些发现的一种可能解释是，腺病毒加速了羊瘙痒病病原体的复制。已知有缺陷的细小病毒会得到腺病毒的帮助。在BALB 3T3细胞培养物中，同时感染的小鼠的脾脏（而非仅感染MAdV的小鼠的脾脏）产生了传染性MAdV以及一种或两种尺寸和形状与细小病毒相同的较小病原体。
