Hassan A K, Scott G R
J Comp Pathol. 1986 May;96(3):241-6. doi: 10.1016/0021-9975(86)90044-7.
When bovine embryonic kidneys collected at the Gorgie Abattoir, Edinburgh were examined for evidence of infection with bovine viral diarrhoea virus (BVDV), 11 out of 26 kidneys were found to be positive. A technique that detected the presence of inadvertent BVDV in cell cultures consisted of processing and digesting the kidneys to produce cell suspensions, adding dimethyl sulphoxide and dispersing the suspensions in small aliquots that were stored frozen at - 114 degrees C. One aliquot was cultured and screened for BVDV by indirect immuno-fluorescence and interference tests. Bovine embryonic kidney cells so processed retained their viability and virus susceptibility for 15 to 18 months. Selected stocks of "clean" cells only are then used for vaccine production or diagnostic purposes. The cytopathic NADL strain of BVDV multiplied in naturally infected cell cultures but the titres attained were significantly lower than in "clean" cell cultures.
对爱丁堡戈吉屠宰场收集的牛胚胎肾进行检查，以寻找感染牛病毒性腹泻病毒（BVDV）的证据，结果发现26个肾中有11个呈阳性。检测细胞培养物中意外存在的BVDV的技术包括处理和消化肾脏以产生细胞悬液，添加二甲基亚砜并将悬液分散成小份，然后在-114℃下冷冻保存。取一份小份进行培养，并通过间接免疫荧光和干扰试验筛查BVDV。经过这样处理的牛胚胎肾细胞在15至18个月内保持其活力和对病毒的敏感性。然后仅选择“清洁”细胞株用于疫苗生产或诊断目的。BVDV的细胞病变NADL株在自然感染的细胞培养物中增殖，但达到的滴度明显低于“清洁”细胞培养物中的滴度。
