在高效液相色谱中实现亚秒级对映异构体拆分的路上。
On our way to sub-second separations of enantiomers in high-performance liquid chromatography.
机构信息
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave. 3, 0179 Tbilisi, Georgia.
Department of Medicine, University Campus Bio-Medico of Rome, Via Alvaro del Portillo 21, 00128 Rome, Italy.
出版信息
J Chromatogr A. 2018 Oct 19;1572:37-43. doi: 10.1016/j.chroma.2018.08.027. Epub 2018 Aug 15.
In this study our preliminary attempt for obtaining fast and highly efficient separations of enantiomers in high-performance liquid chromatography with slightly modified state-of-the-art commercial instrumentation is described. In order to reach this goal after careful selection of chiral analytes, the preparation of chiral stationary phase (CSP), mobile phase composition and column dimensions were optimized. The concept of segmented chiral-achiral column was introduced. As the result of these optimizations baseline separation of enantiomers was achieved with the analysis time between 1-2 s.
在这项研究中，我们初步尝试使用经过轻微改进的最先进商业仪器，在高效液相色谱中快速高效地分离对映异构体。为了达到这个目标，我们在仔细选择手性分析物后，优化了手性固定相（CSP）的制备、流动相组成和柱尺寸。引入了分段手性-非手性柱的概念。通过这些优化，实现了对映异构体的基线分离，分析时间在 1-2 秒之间。
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