Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, UNAM, 04510, Coyoacán, Mexico City, Mexico.
Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana, Unidad Cuajimalpa, Av. Vasco de Quiroga 4871, Col. Santa Fe Cuajimalpa, 05348, Mexico City, Mexico.
Sci Rep. 2018 Aug 23;8(1):12712. doi: 10.1038/s41598-018-31168-0.
Microbiomes influence plant establishment, development, nutrient acquisition, pathogen defense, and health. Plant microbiomes are shaped by interactions between the microbes and a selection process of host plants that distinguishes between pathogens, commensals, symbionts and transient bacteria. In this work, we explore the microbiomes through massive sequencing of the 16S rRNA genes of microbiomes two Marchantia species of liverworts. We compared microbiomes from M. polymorpha and M. paleacea plants collected in the wild relative to their soils substrates and from plants grown in vitro that were established from gemmae obtained from the same populations of wild plants. Our experimental setup allowed identification of microbes found in both native and in vitro Marchantia species. The main OTUs (97% identity) in Marchantia microbiomes were assigned to the following genera: Methylobacterium, Rhizobium, Paenibacillus, Lysobacter, Pirellula, Steroidobacter, and Bryobacter. The assigned genera correspond to bacteria capable of plant-growth promotion, complex exudate degradation, nitrogen fixation, methylotrophs, and disease-suppressive bacteria, all hosted in the relatively simple anatomy of the plant. Based on their long evolutionary history Marchantia is a promising model to study not only long-term relationships between plants and their microbes but also the transgenerational contribution of microbiomes to plant development and their response to environmental changes.
微生物组影响植物的建立、发育、养分获取、病原体防御和健康。植物微生物组是由微生物之间的相互作用和宿主植物的选择过程塑造的，该过程区分了病原体、共生体、共生体和瞬态细菌。在这项工作中，我们通过对两种地钱属（Marchantia）叶状体植物的微生物组 16S rRNA 基因进行大规模测序来探索微生物组。我们比较了野生采集的地钱属植物（M. polymorpha 和 M. paleacea）及其土壤基质的微生物组，以及从同一野生植物种群获得的芽胞在体外培养的植物的微生物组。我们的实验设计允许识别在本地和体外地钱属物种中都发现的微生物。地钱属微生物组的主要 OTUs（97%同一性）被分配到以下属：甲基杆菌属、根瘤菌属、芽孢杆菌属、赖氨酸杆菌属、Pirellula 属、甾醇杆菌属和Bryobacter 属。分配的属对应于能够促进植物生长、复杂分泌物降解、固氮、甲基营养菌和抑制疾病的细菌，所有这些都存在于植物相对简单的解剖结构中。基于其漫长的进化历史，地钱属是一个很有前途的模型，不仅可以研究植物与其微生物之间的长期关系，还可以研究微生物组对植物发育的跨代贡献及其对环境变化的反应。
