通过DNA-DNA和RNA-DNA杂交技术检测尿液中的人巨细胞病毒。

Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridization.

作者信息

Schuster V, Matz B, Wiegand H, Traub B, Kampa D, Neumann-Haefelin D

出版信息

J Infect Dis. 1986 Aug;154(2):309-14. doi: 10.1093/infdis/154.2.309.

DOI:10.1093/infdis/154.2.309

Abstract

A diagnostic hybridization assay for detecting human cytomegalovirus (HCMV) DNA in urine specimens was developed by using cloned viral DNA and in vitro-synthesized RNA probes. Both probes detected 3 pg of homologous DNA and hybridized with DNA of HCMV but not with other viral or human cellular DNA tested. In 95 urine specimens simultaneously tested by cell culture, the sensitivity of hybridization was at least 83%, and the specificity was at least 92%. This assay will be useful for rapid viral diagnosis with wide clinical applications such as screening of immunocompromised patients and quantitation of viral shedding in patients with primary or reactivated HCMV infection who may be receiving antiviral therapy.

摘要

利用克隆的病毒DNA和体外合成的RNA探针，开发了一种用于检测尿液标本中人类巨细胞病毒（HCMV）DNA的诊断杂交试验。两种探针都能检测到3 pg的同源DNA，并与HCMV的DNA杂交，但不与所检测的其他病毒或人类细胞DNA杂交。在同时通过细胞培养检测的95份尿液标本中，杂交的敏感性至少为83%，特异性至少为92%。该试验将有助于快速进行病毒诊断，具有广泛的临床应用，如对免疫功能低下患者的筛查，以及对可能正在接受抗病毒治疗的原发性或再激活HCMV感染患者的病毒排出量进行定量。