Chou S, Merigan T C
N Engl J Med. 1983 Apr 21;308(16):921-5. doi: 10.1056/NEJM198304213081603.
We detected cytomegalovirus DNA in clinical urine specimens after immobilization on nitrocellulose filters and hybridization with a radioactively labeled, cloned fragment of cytomegalovirus DNA. We accomplished the specific detection and quantitation of viral DNA within 24 hours with 39 urine specimens from nine patients with cytomegalovirus viruria, mostly at a tissue-culture infective titer of 10(3) per milliliter or higher. None of 57 urine specimens from 21 patients that were culture-negative for cytomegalovirus gave false-positive results. Analysis of specimens from patients with cytomegalovirus viruria showed a correlation of the infective titer with the intensity of DNA hybridization (r = 0.77). Hybridization of sequential urine specimens from a patient undergoing treatment with interferon for cytomegalovirus retinitis revealed quantitative variations in hybridizable viral DNA over a period that correlated with clinical findings. This assay can be useful in the selection of patients for antiviral therapy and for the assessment of its efficacy.
我们将临床尿液标本固定在硝酸纤维素滤膜上，并用放射性标记的巨细胞病毒DNA克隆片段进行杂交，从而检测其中的巨细胞病毒DNA。我们用来自9例巨细胞病毒血尿症患者的39份尿液标本，在24小时内完成了病毒DNA的特异性检测和定量分析，多数标本的组织培养感染滴度为每毫升10(3)或更高。21例巨细胞病毒培养阴性患者的57份尿液标本均未出现假阳性结果。对巨细胞病毒血尿症患者的标本分析显示，感染滴度与DNA杂交强度相关（r = 0.77）。对一名接受干扰素治疗巨细胞病毒性视网膜炎患者的连续尿液标本进行杂交分析，结果显示在一段时间内可杂交病毒DNA的定量变化与临床结果相关。该检测方法有助于选择抗病毒治疗的患者并评估其疗效。
