Spector S A, Rua J A, Spector D H, McMillan R
J Infect Dis. 1984 Jul;150(1):121-6. doi: 10.1093/infdis/150.1.121.
A diagnostic assay has been developed to detect human cytomegalovirus (HCMV) DNA in clinical specimens with 32P-labeled cloned fragments of HCMV strain AD169. The labeled probe can detect 10 pg of HCMV DNA and fails to hybridize to DNA from other herpesviruses or human DNA. The assay correctly identified 22 (92%) of 24 coded urine specimens culture positive for HCMV and 23 (88%) of 26 urine specimens culture negative for HCMV. In a prospective study of 67 buffy-coat specimens from recipients of bone marrow transplants HCMV DNA was detected in 13 (93%) of 14 culture-positive samples. Of 53 buffy-coat specimens culture negative for HCMV, 32 were hybridization negative for HCMV DNA. However, in 20 of 21 buffy-coat specimens positive for HCMV by hybridization but negative by culture there was evidence that the hybridization assay was correct and more sensitive than currently available tissue culture techniques.
已开发出一种诊断检测方法，可利用人巨细胞病毒（HCMV）AD169株的32P标记克隆片段检测临床标本中的HCMV DNA。标记探针可检测到10 pg的HCMV DNA，且不会与其他疱疹病毒的DNA或人类DNA杂交。该检测方法正确鉴定出24份经编码的HCMV培养阳性尿液标本中的22份（92%），以及26份HCMV培养阴性尿液标本中的23份（88%）。在一项对67份骨髓移植受者的血沉棕黄层标本进行的前瞻性研究中，在14份培养阳性样本中的13份（93%）检测到了HCMV DNA。在53份HCMV培养阴性的血沉棕黄层标本中，32份HCMV DNA杂交阴性。然而，在21份通过杂交检测HCMV呈阳性但培养呈阴性的血沉棕黄层标本中，有20份有证据表明杂交检测是正确的，且比目前可用的组织培养技术更敏感。
