Meng Qingfeng, Gupta Ravi, Kwon Soon Jae, Wang Yiming, Agrawal Ganesh Kumar, Rakwal Randeep, Park Sang-Ryeol, Kim Sun Tae
Department of Plant Bioscience, Life and Energy Convergence Research Institute, Pusan National University, Miryang 46241, Korea.
Department of Plant-Microbe Interactions, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, 50829 Cologne, Germany.
Plant Pathol J. 2018 Aug;34(4):257-268. doi: 10.5423/PPJ.OA.01.2018.0008. Epub 2018 Aug 1.
Rice blast disease, caused by , results in an extensive loss of rice productivity. Previously, we identified a novel secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes ( < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice.
稻瘟病由[病原体名称未给出]引起，会导致水稻产量大幅损失。此前，我们鉴定出一种新型分泌蛋白，称为MSP1，它会在水稻中引发细胞死亡和病原体相关分子模式（PAMP）触发的免疫（PTI）反应。在此，我们报告了水稻中MSP1诱导反应的转录组概况，这使得我们鉴定出21,619个基因，其中4,386个基因在外源MSP1处理后表现出显著变化（<0.05且变化倍数>2或<1/2）。对差异调节基因的功能注释表明，被抑制的基因与光合作用、次生代谢、脂质合成和蛋白质合成密切相关，而诱导基因则参与脂质降解、蛋白质降解和信号传导。此外，编码类受体激酶、丝裂原活化蛋白激酶（MAPK）、WRKY转录因子、激素信号蛋白和病程相关（PR）蛋白的基因表达也受到MSP1的诱导。将这些差异表达基因映射到各种途径上揭示了有关MSP1触发反应的关键信息，为水稻中MSP1触发的PTI反应的分子机制和组成成分提供了新的见解。
