Liu Huan, Chen Zheng-Shi, Zhang Dong-Ju, Lu Yong-Yue
Department of Entomology, South China Agricultural University, Guangzhou, China.
Front Physiol. 2018 Jul 26;9:987. doi: 10.3389/fphys.2018.00987. eCollection 2018.
Insect attractants are important prevention tools for managing populations of the Oriental fruit fly, (Hendel), which is a highly destructive agricultural pest with health implications in tropical and subtropical countries. Methyl eugenol (ME) is still considered the gold standard of attractants. Mature male flies use their olfactory system to detect ME, but the molecular mechanism underlying their olfactory detection of ME largely remains unclear. Here, we showed that ME activates the odorant receptors and in mature males antennae by RNA-Seq and qRT-PCR analysis. Interestingly, ME only elicited robust responses in the /-expressing oocytes, thus suggesting that is necessary for ME reception and tropism in . Next, our indoor behavioral assays demonstrated that knockdown had no significant effects on ME detection and tropism. By contrast, reducing the transcript levels led to a significant decrease in the males' responsiveness to ME. Taken together, our results gave novel insight in the understanding of the olfactory background to the Oriental fruit fly's attraction toward ME.
昆虫引诱剂是管理东方果实蝇（亨德尔）种群的重要防治工具，东方果实蝇是一种极具破坏性的农业害虫，在热带和亚热带国家对健康也有影响。甲基丁香酚（ME）仍然被认为是引诱剂的黄金标准。成熟雄蝇利用其嗅觉系统来检测ME，但其对ME嗅觉检测的分子机制在很大程度上仍不清楚。在这里，我们通过RNA测序和定量逆转录聚合酶链反应分析表明，ME在成熟雄蝇触角中激活了气味受体和。有趣的是，ME仅在表达的卵母细胞中引发强烈反应，因此表明对于ME在中的接收和向性是必要的。接下来，我们的室内行为分析表明，敲低对ME检测和向性没有显著影响。相比之下，降低转录水平导致雄蝇对ME的反应性显著降低。综上所述，我们的结果为理解东方果实蝇对ME的吸引力的嗅觉背景提供了新的见解。
