Li Weiwei, Khan Mashooq, Mao Sifeng, Feng Shuo, Lin Jin-Ming
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
J Pharm Anal. 2018 Aug;8(4):210-218. doi: 10.1016/j.jpha.2018.07.005. Epub 2018 Jul 17.
The metastasis in which the cancer cells degrade the extracellular matrix (ECM) and invade to the surrounding and far tissues of the body is the leading cause of mortality in cancer patients. With a lot of advancement in the field, yet the biological cause of metastasis are poorly understood. The microfluidic system provides advanced technology to reconstruct a variety of in vivo-like environment for studying the interactions between tumor cells (TCs) and endothelial cells (ECs). This review gives a brief account of both two-dimensional models and three-dimensional microfluidic systems for the analysis of TCs-ECs co-culture as well as their applications to anti-cancer drug screening. Furthermore, the advanced methods for analyzing cell-to-cell interactions at single-cell level were also discussed.
癌细胞降解细胞外基质（ECM）并侵入身体周围和远处组织的转移是癌症患者死亡的主要原因。尽管该领域取得了很大进展，但转移的生物学原因仍知之甚少。微流控系统提供了先进技术，可重建各种类似体内的环境，用于研究肿瘤细胞（TCs）与内皮细胞（ECs）之间的相互作用。本文综述简要介绍了用于分析TCs-ECs共培养的二维模型和三维微流控系统，以及它们在抗癌药物筛选中的应用。此外，还讨论了在单细胞水平分析细胞间相互作用的先进方法。
