Su Chong, Liu Yingze, He Yang, Gu Jingkai
College of Life Science, Jilin University, Changchun 130012, PR China.
Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of lowa, lowa City, United States.
J Pharm Anal. 2018 Aug;8(4):219-225. doi: 10.1016/j.jpha.2018.07.002. Epub 2018 Jul 4.
Nanoliposomes are considered to be the most successful nanoparticle drug delivery system, but their fate in vivo has not been fully understood due to lack of reliable bioanalytical methods, which seriously limits the development of liposomal drugs. Hence, an overview of currently used bioanalytical methods is imperative to lay the groundwork for the need of developing a bioanalytical method for liposome measurements in vivo. Currently, major analytical methods for nanoliposomes measurement in vivo include fluorescence labeling, radiolabeling, magnetic resonance imaging (MRI), mass spectrometry and computed tomography. In this review, these bioanalytical methods are summarized, and the advantages and disadvantages of each are discussed. We provide insights into the applicability and limitations of these analytical methods in the application of nanoliposomes measurement in vivo, and highlight the recent development of instrumental analysis techniques. The review is devoted to providing a comprehensive overview of the investigation of nanoliposomes design and associated fate in vivo, promoting the development of bioanalytical techniques for nanoliposomes measurement, and understanding the pharmacokinetic behavior, effectiveness and potential toxicity of nanoliposomes in vivo.
纳米脂质体被认为是最成功的纳米颗粒药物递送系统，但由于缺乏可靠的生物分析方法，其在体内的命运尚未得到充分了解，这严重限制了脂质体药物的发展。因此，概述当前使用的生物分析方法对于为开发体内脂质体测量的生物分析方法奠定基础至关重要。目前，体内纳米脂质体测量的主要分析方法包括荧光标记、放射性标记、磁共振成像（MRI）、质谱和计算机断层扫描。在本综述中，对这些生物分析方法进行了总结，并讨论了每种方法的优缺点。我们深入探讨了这些分析方法在体内纳米脂质体测量应用中的适用性和局限性，并突出了仪器分析技术的最新进展。本综述致力于全面概述纳米脂质体设计及其在体内相关命运的研究，促进纳米脂质体测量生物分析技术的发展，并了解纳米脂质体在体内的药代动力学行为、有效性和潜在毒性。
