Azim A, Ahmed S, Paul S K, Nasreen S A, Sarkar S R, Ahmed M U, Najnin A, Hossain M A
Dr Anuwarul Azim, PG (M Phil) Student, Department of Microbiology, Mymensingh Medical College (MMC), Bangladesh; E-mail:
Mymensingh Med J. 2018 Jul;27(3):440-444.
Uncooked vegetables are an important part of a healthy diet in different parts of the world. The present descriptive cross sectional type of study was carried out to determine the parasitological contamination of vegetables sold at markets of Mymensingh city of Bangladesh. Parasitological examinations were performed in material derived from 200 specimens of 10 different vegetables to detect intestinal parasites in the department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh from July 2015 to July 2016. Each sample was washed with 5 liter distilled water, then washing fluid was centrifuged and resulting sediment was collected for iodine and normal saline wet mount slide preparation to detect human pathogenic intestinal parasites by microscopic examination. Out of 200 vegetable samples, 123(61.5%) were contaminated with different species of parasites. The vegetables red amaranth had the highest contamination rate of 18/20 (90%) followed by jute leaf 17/20 (85%), coriander leaf 15/20 (75%), onion 14/20 (70%), lady's finger 14/20 (70%), radish 13/20 (65%), green pepper 12/20 (60%), carrot 12/20 (60%), cucumber 5/20 (25%), tomato 3/20 (15%). Among the contaminating parasites Ascaris lumbricoides (egg) 36.5% was the most prevalent followed by Strongyloides stercoralis (larva) 35.5%, Entamoeba histolytica ( trophozoite and different cystic stages) 8.5%, Hook worm (egg and larva) 6.5% , Balantidium coli (trophozoite) 4.0%, Enterobius vermicularis (egg) 3.5%, Trichuris trichiura (egg) 1.5%, Giardia lamblia (cyst) 1.0%. To our knowledge, it was the first base line study pursued in Bangladesh. Contamination of raw vegetables from markets with pathogenic parasites would increase the risk of disease to the population that consumes or works with these products. So improperly washed raw eaten vegetables should be considered a potential risk for contracting parasites, particularly helminthes in Mymensingh city.
生蔬菜是世界不同地区健康饮食的重要组成部分。本研究采用描述性横断面研究类型，以确定孟加拉国迈门辛市市场上销售的蔬菜的寄生虫污染情况。2015年7月至2016年7月期间，在孟加拉国迈门辛医学院微生物学系，对来自10种不同蔬菜的200个样本进行了寄生虫学检查，以检测肠道寄生虫。每个样本用5升蒸馏水冲洗，然后将冲洗液离心，收集所得沉淀物用于制备碘液和生理盐水湿片，通过显微镜检查检测人体致病性肠道寄生虫。在200个蔬菜样本中，123个（61.5%）被不同种类的寄生虫污染。红苋菜的污染率最高，为18/20（90%），其次是黄麻叶17/20（85%）、香菜叶15/20（75%）、洋葱14/20（70%）、秋葵14/20（70%）、萝卜13/20（65%）、青椒12/20（60%）、胡萝卜12/20（60%）、黄瓜5/20（25%）、番茄3/20（15%）。在污染的寄生虫中，蛔虫（卵）最为普遍，占36.5%，其次是粪类圆线虫（幼虫）35.5%、溶组织内阿米巴（滋养体和不同囊泡阶段）8.5%、钩虫（卵和幼虫）6.5%、结肠小袋纤毛虫（滋养体）4.0%、蛲虫（卵）3.5%、鞭虫（卵）1.5%、蓝氏贾第鞭毛虫（包囊）1.0%。据我们所知，这是在孟加拉国进行的第一项基线研究。市场上的生蔬菜被致病性寄生虫污染会增加食用或接触这些产品的人群患病的风险。因此，在迈门辛市，未经适当清洗就生食的蔬菜应被视为感染寄生虫，尤其是蠕虫的潜在风险因素。
