Osman Ghenijan, Wang Zhifang, Wang Ning, Shayimu Gulinisha, Hou Min, Guo Wenchao, Yang Xinping
1Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, PR China.
2Xingjian Laboratory of Special Environmental Microbiology, Urumqi, 830091, PR China.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3243-3247. doi: 10.1099/ijsem.0.002972. Epub 2018 Aug 24.
Strain XAAS-R86, a Gram-stain-negative, short rod-shaped, non-motile, aerobic bacterium, was isolated from a Populus euphratica forest near the city of Hotan, Xinjiang, PR China. The cells were found to be positive for catalase and oxidase activities. Growth occurred optimally at 28-30 °C, pH 7.0-7.5 and in the presence of 0.5-2.0 % NaCl (w/v). The results of phylogenetic analysis of the 16S rRNA gene indicated that XAAS-R86 represents a member of the genus Pontibacter within the family Hymenobacteraceae. Pontibacter akesuensis CCTCC AB 206086 is the most closely related species with a validly published name, based on 16S rRNA gene sequence identity (95.7 %). The DNA G+C content of the strain is 43.9 mol%. The main respiratory quinone is MK-7 and the major cellular fatty acids are summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B) and iso-C15 : 0 and its major polar lipids are phosphatidylethanolamine and two unidentified lipids. On the basis of the results of tests performed using a polyphasic approach, XAAS-R86 represents a novel species of the genus Pontibacter, for which the name Pontibactersilvestris sp. nov. is proposed, with the type strain XAAS-R86 (=CCTCC AB 2017165=KCTC 62047).
菌株XAAS-R86是一种革兰氏阴性、短杆状、无运动性的需氧细菌，从中国新疆和田市附近的胡杨林中分离得到。发现该细胞过氧化氢酶和氧化酶活性呈阳性。在28-30 °C、pH 7.0-7.5以及存在0.5-2.0 %（w/v）NaCl的条件下生长最佳。16S rRNA基因的系统发育分析结果表明，XAAS-R86代表了膜杆菌科Pontibacter属的一个成员。基于16S rRNA基因序列同一性（95.7 %），阿克苏Pontibacter菌CCTCC AB 206086是与其最密切相关的有效发表种。该菌株的DNA G+C含量为43.9 mol%。主要呼吸醌为MK-7，主要细胞脂肪酸为总和特征4（异-C17 : 1I和/或anteiso-C17 : 1B）和异-C15 : 0，其主要极性脂质为磷脂酰乙醇胺和两种未鉴定的脂质。基于多相方法的测试结果，XAAS-R86代表Pontibacter属的一个新物种，为此提出Pontibactersilvestris sp. nov.这个名称，模式菌株为XAAS-R86（=CCTCC AB 2017165=KCTC 62047）。
