Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005058.
A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6 was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea. Cells of SD6 grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %). Moreover, 16S rRNA gene sequence analysis indicated that strain SD6 was a member of the genus , sharing similarities to XAAS-1 (98.0 %), 9-2 (97.3 %), X14-1 (97.2 %) and HYL7-26 (96.8 %). The predominant fatty acids of strain SD6 were identified as iso-C and summed feature 4 (comprising anteiso-C B and/or iso-C I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7). Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid. The average nucleotide identity and DNA-DNA hybridization values of strain SD6 with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively. The genomic DNA G+C content was 45.4 mol%. In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6 represents a novel species of the genus r, for which the name sp. nov. is proposed. The type strain is SD6 (=KACC 21543=NBRC 114313=JCM 31022).
一株革兰氏阴性、杆状、非运动、粉红色的细菌，被命名为 SD6，从韩国郁陵岛 Sadong 海滩的沿海水域中分离得到。SD6 细胞在 10-42°C（最适温度 30°C）、pH5.0-9.0（最适 pH6.0-7.0）和 0-8.0%（w/v）NaCl（最适 0-3%NaCl）的条件下生长。此外，16S rRNA 基因序列分析表明，该菌株属于 属，与 XAAS-1（98.0%）、9-2（97.3%）、X14-1（97.2%）和 HYL7-26（96.8%）最为相似。该菌株的主要脂肪酸为 iso-C 和 sumFeature 4（包括 anteiso-C B 和/或 iso-C I），唯一的呼吸醌为 MK-7（menaquinone 7）。主要的极性脂包括磷脂酰乙醇胺、一种未鉴定的磷酸甘油酯、两种未鉴定的糖脂和一种未鉴定的脂类。该菌株与亲缘关系最近的菌株的平均核苷酸同一性和 DNA-DNA 杂交值分别为 72.8-79.8%和 19.2-22.6%。基因组 DNA G+C 含量为 45.4 mol%。根据表型、化学分类和系统发育数据的结果，菌株 SD6 代表了 属的一个新种，提议将其命名为 sp. nov.。其模式菌株为 SD6（=KACC 21543=NBRC 114313=JCM 31022）。
