最小异手性从头设计的 4Fe-4S 结合肽，能够进行稳健的电子转移。
Minimal Heterochiral de Novo Designed 4Fe-4S Binding Peptide Capable of Robust Electron Transfer.
机构信息
Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers , the State University of New Jersey , New Brunswick , New Jersey 08901 , United States.
Center for Advanced Biotechnology and Medicine , Rutgers, the State University of New Jersey , Piscataway , New Jersey 08854 , United States.
出版信息
J Am Chem Soc. 2018 Sep 12;140(36):11210-11213. doi: 10.1021/jacs.8b07553. Epub 2018 Aug 29.
Abstract
Ambidoxin is a designed, minimal dodecapeptide consisting of alternating L and D amino acids that binds a 4Fe-4S cluster through ligand-metal interactions and an extensive network of second-shell hydrogen bonds. The peptide can withstand hundreds of oxidation-reduction cycles at room temperature. Ambidoxin suggests how simple, prebiotic peptides may have achieved robust redox catalysis on the early Earth.
摘要
两性霉素是一种设计的、最小的十二肽，由交替的 L 和 D 氨基酸组成，通过配体-金属相互作用和广泛的第二壳层氢键网络结合 4Fe-4S 簇。该肽在室温下可以承受数百个氧化还原循环。两性霉素表明，简单的、前生物肽可能在早期地球上实现了强大的氧化还原催化作用。
相似文献
1
Minimal Heterochiral de Novo Designed 4Fe-4S Binding Peptide Capable of Robust Electron Transfer.最小异手性从头设计的 4Fe-4S 结合肽，能够进行稳健的电子转移。
J Am Chem Soc. 2018 Sep 12;140(36):11210-11213. doi: 10.1021/jacs.8b07553. Epub 2018 Aug 29.
2
Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase.调节[4Fe-4S]中心的氧化还原性质以调控钼双PGD周质硝酸还原酶的活性。
Biochim Biophys Acta Bioenerg. 2019 May 1;1860(5):402-413. doi: 10.1016/j.bbabio.2019.01.003. Epub 2019 Jan 29.
3
Modified ligands to FA and FB in photosystem I. II. Characterization of a mixed ligand [4Fe-4S] cluster in the C51D mutant of PsaC upon rebinding to P700-Fx cores.光系统I中对FA和FB的修饰配体。II. PsaC的C51D突变体中混合配体[4Fe-4S]簇与P700-Fx核心重新结合后的表征。
J Biol Chem. 1995 Nov 24;270(47):28118-25. doi: 10.1074/jbc.270.47.28118.
4
Modulation of cluster incorporation specificity in a de novo iron-sulfur cluster binding peptide.从头合成铁硫簇结合肽中簇掺入特异性的调控
Biopolymers. 2015 Jul;104(4):412-8. doi: 10.1002/bip.22635.
5
Protonation/reduction dynamics at the [4Fe-4S] cluster of the hydrogen-forming cofactor in [FeFe]-hydrogenases.[铁铁]氢化酶中氢形成辅因子的[4Fe-4S]簇处的质子化/还原动力学。
Phys Chem Chem Phys. 2018 Jan 31;20(5):3128-3140. doi: 10.1039/c7cp04757f.
6
Proton coupling to [4Fe-4S](2+/+) and [4Fe-4Se](2+/+) oxidation and reduction in a designed protein.质子与设计蛋白中[4Fe-4S](2+/+)和[4Fe-4Se](2+/+)的氧化还原偶联
J Am Chem Soc. 2002 Jun 19;124(24):6826-7. doi: 10.1021/ja0171613.
7
The function and properties of the iron-sulfur center in spinach ferredoxin: thioredoxin reductase: a new biological role for iron-sulfur clusters.菠菜铁氧化还原蛋白：硫氧还蛋白还原酶中铁硫中心的功能与特性：铁硫簇的一种新生物学作用
Biochemistry. 1996 Sep 3;35(35):11425-34. doi: 10.1021/bi961007p.
8
Synthesis and characterization of de novo designed peptides modelling the binding sites of [4Fe-4S] clusters in photosystem I.
Biochim Biophys Acta. 2009 Aug;1787(8):995-1008. doi: 10.1016/j.bbabio.2009.03.007. Epub 2009 Mar 16.
9
The iron-sulfur clusters in Escherichia coli succinate dehydrogenase direct electron flow.大肠杆菌琥珀酸脱氢酶中的铁硫簇引导电子流动。
J Biol Chem. 2006 Sep 15;281(37):27662-8. doi: 10.1074/jbc.M604900200. Epub 2006 Jul 23.
10
Probing ligand effects on the redox energies of [4Fe-4S] clusters using broken-symmetry density functional theory.使用破缺对称性密度泛函理论探究配体对[4Fe-4S]簇氧化还原能的影响。
J Phys Chem A. 2009 May 14;113(19):5671-6. doi: 10.1021/jp809446q.
引用本文的文献
1
RNA binding and coacervation promote preservation of peptide form and function across the heterochiral-homochiral divide.RNA结合与凝聚促进了肽的形式和功能在异手性-同手性分界间的保存。
Protein Sci. 2025 Sep;34(9):e70273. doi: 10.1002/pro.70273.
2
On the emergence of metabolism: the evolution of proteins that powered life.论新陈代谢的出现：驱动生命的蛋白质的进化
Philos Trans R Soc Lond B Biol Sci. 2025 Aug 7;380(1931):20240090. doi: 10.1098/rstb.2024.0090.
3
Bottom-up design of peptide shapes in water using oligomers of -methyl-l/d-alanine.利用α-甲基-L/D-丙氨酸的低聚物在水中进行肽形状的自下而上设计。
Chem Sci. 2025 May 2. doi: 10.1039/d5sc01483b.
4
Shedding Light on the Electron Delocalization Pathway at the [FeS] Cluster of FDX2.揭示FDX2的[FeS]簇处的电子离域途径
Inorg Chem. 2025 Apr 7;64(13):6698-6712. doi: 10.1021/acs.inorgchem.5c00420. Epub 2025 Mar 23.
5
Recent advances in de novo designed metallopeptides as tailored enzyme mimics.作为定制酶模拟物的从头设计金属肽的最新进展。
Curr Opin Chem Biol. 2025 Jun;86:102586. doi: 10.1016/j.cbpa.2025.102586. Epub 2025 Mar 20.
6
Redefining the Limits of Functional Continuity in the Early Evolution of P-Loop NTPases.重新定义P环NTP酶早期进化中功能连续性的界限。
Mol Biol Evol. 2025 Apr 1;42(4). doi: 10.1093/molbev/msaf055.
7
Regulating ferredoxin electron transfer using nanobody and antigen interactions.利用纳米抗体与抗原的相互作用调控铁氧化还原蛋白的电子转移
RSC Chem Biol. 2025 Jan 31;6(5):746-753. doi: 10.1039/d4cb00257a. eCollection 2025 May 8.
8
Prebiotic synthesis of the major classes of iron-sulfur clusters.铁硫簇主要类别前体的合成
Chem Sci. 2025 Feb 11;16(11):4614-4624. doi: 10.1039/d5sc00524h. eCollection 2025 Mar 12.
9
Bottom-up construction of chiral metal-peptide assemblies from metal cluster motifs.从金属团簇基元出发，通过自下而上的方法构建手性金属-肽组装体。
Nat Commun. 2024 Oct 19;15(1):9034. doi: 10.1038/s41467-024-53320-3.
10
Protocellular Heme and Iron-Sulfur Clusters.原核细胞血红素和铁硫簇
Acc Chem Res. 2024 Aug 20;57(16):2293-2302. doi: 10.1021/acs.accounts.4c00254. Epub 2024 Aug 5.
本文引用的文献
1
Modular origins of biological electron transfer chains.生物电子传递链的模块化起源。
Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1280-1285. doi: 10.1073/pnas.1714225115. Epub 2018 Jan 22.
2
SCOPe: Manual Curation and Artifact Removal in the Structural Classification of Proteins - extended Database.SCOPe：蛋白质结构分类中的人工整理与伪迹去除——扩展数据库
J Mol Biol. 2017 Feb 3;429(3):348-355. doi: 10.1016/j.jmb.2016.11.023. Epub 2016 Nov 30.
3
The Role of Microbial Electron Transfer in the Coevolution of the Biosphere and Geosphere.微生物电子转移在生物圈和岩石圈协同进化中的作用。
Annu Rev Microbiol. 2016 Sep 8;70:45-62. doi: 10.1146/annurev-micro-102215-095521. Epub 2016 Jun 8.
4
Structural principles for computational and de novo design of 4Fe-4S metalloproteins.4Fe-4S金属蛋白计算与从头设计的结构原理
Biochim Biophys Acta. 2016 May;1857(5):531-538. doi: 10.1016/j.bbabio.2015.10.001. Epub 2015 Oct 9.
5
Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.在线粒体铁硫簇装配机器中形成 [4Fe-4S] 簇。
J Am Chem Soc. 2014 Nov 19;136(46):16240-50. doi: 10.1021/ja507822j. Epub 2014 Nov 7.
6
Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.含有细胞色素、铁硫或铜氧化还原中心的金属蛋白。
Chem Rev. 2014 Apr 23;114(8):4366-469. doi: 10.1021/cr400479b.
7
De novo design of an artificial bis[4Fe-4S] binding protein.从头设计人工双[4Fe-4S]结合蛋白。
Biochemistry. 2013 Oct 29;52(43):7586-94. doi: 10.1021/bi401199s. Epub 2013 Oct 18.
8
Energetic selection of topology in ferredoxins.在[铁氧还蛋白]中对拓扑结构进行的能量选择。
PLoS Comput Biol. 2012;8(4):e1002463. doi: 10.1371/journal.pcbi.1002463. Epub 2012 Apr 5.
9
Empirical and computational design of iron-sulfur cluster proteins.铁硫簇蛋白的经验性与计算性设计
Biochim Biophys Acta. 2012 Aug;1817(8):1256-62. doi: 10.1016/j.bbabio.2012.02.001. Epub 2012 Feb 8.
10
History of biological metal utilization inferred through phylogenomic analysis of protein structures.通过蛋白质结构的系统基因组学分析推断生物金属利用的历史。
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10567-72. doi: 10.1073/pnas.0912491107. Epub 2010 May 24.
Zotero 插件