Universidade Federal Rural de Pernambuco - UFRPE, Recife, PE, Brazil.
Universidade Federal Rural de Pernambuco - UFRPE, Recife, PE, Brazil.
Microb Pathog. 2018 Nov;124:163-169. doi: 10.1016/j.micpath.2018.08.029. Epub 2018 Aug 22.
In the Northeast of Brazil, ethnoveterinary studies have shown that the body fat from Gallus gallus domesticus and Meleagris gallopavo are used for diseases that affect domestic animals. The objective of this study was to identify the chemical composition and to evaluate the antibacterial activity of the Gallus gallus domesticus (OFGG) and Meleagris gallopavo (OFMG) fixed oils in isolation and in association with antibiotics. The OFGG and OFMG from the poultry's body fat were extracted using hexane as a solvent in Soxhlet. Their composition was indirectly determined using fatty acid methyl esters. The OFGG and OFMG antibacterial and modulatory activities against standard and multi-resistant bacterial strains were performed through the broth microdilution test. In the OFGG chemical composition, 4 constituents were identified. The saturated fatty acid (AGS) and unsaturated fatty acid (AGI) percentages were 35.1% and 64.91% respectively, with linoleic acid being the major component. In the OFMG, 3 constituents were identified. The AGS percentage was 27.71% and 72.29% for AGI, with oleic acid as the most abundant component. The oils did not present antibacterial activity when tested in isolation, presenting Minimum Inhibitory Concentrations (MICs) > 512 μg/mL. However, when associated with antibiotics the OFGG showed synergistic activity with the antibiotics Amikacin, Amoxicillin, Norfloxacin and Oxytetracycline, while the OFMG promoted a synergistic action with the antibiotics Amikacin, Amoxicillin and Norfloxacin.
在巴西东北部，民族兽医研究表明，家鸡和火鸡的体脂被用于治疗影响家畜的疾病。本研究的目的是鉴定化学组成，并评估家鸡（OFGG）和火鸡（OFMG）固定油的分离和与抗生素联合的抗菌活性。使用正己烷作为溶剂，在索氏提取器中从家禽体脂中提取 OFGG 和 OFMG。使用脂肪酸甲酯间接确定它们的组成。通过肉汤微量稀释试验测定 OFGG 和 OFMG 对标准和多耐药菌株的抗菌和调节活性。在 OFGG 的化学成分中，鉴定出 4 种成分。饱和脂肪酸（AGS）和不饱和脂肪酸（AGI）的百分比分别为 35.1%和 64.91%，其中亚油酸是主要成分。在 OFMG 中，鉴定出 3 种成分。AGS 的百分比为 27.71%，AGI 的百分比为 72.29%，其中油酸的含量最多。单独测试时，这些油没有抗菌活性，最低抑菌浓度（MIC）均大于 512 μg/mL。然而，当与抗生素联合使用时，OFGG 显示与抗生素阿米卡星、阿莫西林、诺氟沙星和土霉素具有协同作用，而 OFMG 促进了抗生素阿米卡星、阿莫西林和诺氟沙星的协同作用。
